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Lipids

Lipids and fatty acid analytical standards

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Lipids and fatty acids are analyzed in a wide variety of industries, including food testing, clinical and biomedical testing, microbiological testing, and more. Agilent offers a wide range of lipids and fatty acid analytical standards including, but not limited to, fatty acid methyl esters and fatty alcohols, as well as fatty acid and methyl ester kits that provide a variety of compounds.

Bond Elut EMR-Lipid

Bond Elut Enhanced Matrix Removal – Lipid is designed to extract lipids from high-fat samples.

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Bond Elut Enhanced Matrix Removal - Lipid provides the most comprehensive matrix removal of any sample preparation product. Lipids can be extracted selectively from high-fat samples without analyte loss. The final polishing step removes excess water and improves analyte partitioning.|These QuEChERS kits contain 1 g of preweighed sorbent in 15 mL centrifuge tubes. They can easily accommodate the aliquot volumes specified by current AOAC and EN methodologies.

Bond Elut Lipid Extraction

Bond Elut Lipid Extraction SPE 1 mL cartridges and 2 mL 96-well plates provide highly selective and efficient extraction of lipids for lipid analysis, lipid profiling, and lipidomics workflows.

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Bond Elut Lipid Extraction SPE 1 mL cartridges and 2 mL 96-well plates provide highly selective and efficient extraction of lipids for lipid analysis, lipid profiling, and lipidomics workflows. Featuring unique EMR-Lipid sorbent technology, these SPE products combine size exclusion and hydrophobic interactions to exclusively trap and isolate lipids which can then be effectively eluted from the cartridge or well plate. A wash step is implemented in between sample loading and lipid elution to wash off additional matrix interferences.|In lipid analysis, effective sample preparation is critical to isolating lipids from any additional co-extractives. There are significant sacrifices made when using traditional LLE methods, including method reproducibility, researchers' time and effort, and the requirement for more samples to achieve statistically reliable results. With Bond Elut Lipid Extraction 1 mL cartridge and 2 mL 96-well plate SPE formats, you can easily achieve more reproducible and streamlined sample preparation for lipid analysis and reduce the need for precious research samples for evaluation. In addition, the 2 mL 96-well plate format promotes an automated workflow for lipid analysis.

Captiva EMR-Lipid and Captiva EMR-Lipid HF

Enables selective, effective lipid/matrix removal without analyte loss.

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The award-winning Captiva EMR-Lipid provides highly selective, efficient lipid/matrix removal without analyte loss. The novel Enhanced Matrix Removal-Lipid technology removes lipids based on size exclusion and hydrophobic interaction. Effective enhanced matrix removal (EMR) lipid removal ensures minimal ion suppression of target analytes, significantly improving method reliability and ruggedness.|Captiva EMR-Lipid 96-well plate and 1 mL cartridge formats contain a solvent retention frit, which allows in-well protein precipitation that streamlines sample preparation. The improved filter design promotes easy flow with vacuum or positive pressure. Choose Captiva EMR-Lipid 96-well plate or 1 mL cartridge formats for biological sample preparation. |Captiva EMR–Lipid and Captiva EMR–Lipid HF in 3 and 6 mL cartridges allow simple passthrough removal of lipids, fats, and other interferences from complex fatty samples. These formats accommodate larger volumes of complex sample extracts including those from meat, fish, oils, dairy products, and other foods of animal origin. Captiva EMR–Lipid HF is an excellent choice when a vacuum manifold or positive pressure manifold is not available. Designed for high flow (HF) rates using gravity, this product offers carefree, walkaway, gravity elution.|In 2017, Captiva EMR-Lipid won an Analytical Scientist Innovation Award (TASIA).

Captiva ND & ND (Non-Drip) Lipids Cartridges & Plates

ND (non-drip) cartridges and plates enable uniform flow to prevent filter clogging and good filtrate recovery.

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Captiva ND (non-drip) cartridges and plates are a simple-to-use filtration device designed for high-throughput, automated, in-well protein precipitation. With a unique non-drip (ND) membrane, Captiva ND products enable solvent-first protein precipitation using methanol or acetonitrile. The dual-filter design delivers fast, uniform flow, avoiding filter plugging and ensuring reliable filtrate recovery.|In addition, Captiva ND Lipids cartridges and plates are designed to effectively remove phospholipids from biofluids. The removal of lipid compounds results in enhanced sensitivity, better peak shapes, and more reproducible retention times. Captiva ND and ND Lipids products ensure high sample throughput and help to reduce instrumentation downtime in the laboratory.

Community Search Results

Captiva EMR Lipid Solvent Compatibility Captiva EMR Lipid Solvent Compatibility

Greetings, We are interested in trying EMR lipid cartridges for cleanup of nonpolar extracts (targeting PAHs, PCBs, etc.). I understand that the cartridges need to be activated with a certain quantity of water + water-miscible solvent, but are there any concerns regarding performance with stronger solvents such as acetone, hexanes, isooctane, toluene, etc.? I have seen that the efficiency of some other sorbents targeting lipids/fatty acids are harmed by more nonpolar solvents, and acetone in particular (but this was more related to primary-secondary-amine phase interactions rather than the polarity of the solvent per se).

Deconvolution software for peak separation of lipid regioisomers Deconvolution software for peak separation of lipid regioisomers

We are analysing lipid regioisomers and so far we found a chiral column that can do this. However, we need to run the LC isocratically (99.5% acetonitrile, 0.5% 90/10 isopropanol/acetonitrile + 1mM ammonium formate + 0.1% formic acid). Now, we want to reduce the runtime but this causes the peaks to overlap. Because they are lipid regioisomers, they have the same fragmentation pattern, and so that only way for separation is by chromatography. 1. Is there any software that can deconvolute the peaks?

Fractionation of lipids using NH2 SPE Cartridge Fractionation of lipids using NH2 SPE Cartridge

I’m Yutaro, a university student in Japan. I would like to inquire about the classification of lipids using NH2 SPE cartridges. Using Bond Elut NH2 SPE cartridges (500 mg, 3 mL), I'm trying to fractionate lipids into the neutral lipid (NL) and phospholipid (PL) fractions.

Raw Material Identification of mRNA Lipid Nanoparticle Components with the Agilent Vaya Raman Spectrometer Raw Material Identification of mRNA Lipid Nanoparticle Components with the Agilent Vaya Raman Spectrometer

Some COVID-19 vaccines are using lipid-based nanoparticles to act as drug delivery systems for effective on-target payload delivery. Just like any finished product components, lipid nanoparticle components—like pegylated lipids, cholesterol, ionizable cationic lipids, and cryoprotectants—must be verified for ID at receipt before use in vaccine manufacturing. In a recent application note, the Vaya handheld Raman spectrometer , based on spatially offset Raman spectroscopy (SORS) , was shown to probe through opaque and transparent containers and ID the different components used in the making of lipid nanoparticles for easier, low cost ID at receipt—with no sampling, no container opening, and no sampling booth required. Download the application note for full details: Identification of mRNA Lipid Nanoparticle Components with the Agilent Vaya Raman Spectrometer

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