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Explore New Infinity III LC Series
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Set of system capillaries and fittings to ramp up usage of HPLC instruments
InfinityLab HPLC capillary tubing kits are complete sets of system capillary tubing and fittings, including InfinityLab Quick Connect and Quick Turn fittings, to efficiently ramp up usage of InfinityLab LC Series instruments.|Capillary fitting starter kits contain the most widely used connection capillaries, fittings, and tools needed for legacy HPLC systems. InfinityLab capillary kits are complete sets of capillaries and fittings for Infinity II LC systems. InfinityLab convenience kits contain both the contents of the capillary kits and a variety of selected products such as solvent bottles, Stay Safe caps, inline filter, vials, and tools to provide everything you need to start your LC.|In all the kits, capillary dimensions are optimized to minimize extra-column volume to ensure highest separation efficiency.
An adjustable HPLC cart and an LC bench to optimize lab space.
HPLC benches are HPLC tables or carts designed specifically to organize HPLC and UHPLC systems. The Agilent HPLC bench portfolio offers mobile or benchtop solutions for instrument configuration that address the high demand for flexibility in the lab.|The InfinityLab Flex Bench is an adjustable HPLC table on wheels. The flexibility of this HPLC cart makes it safe and easy to relocate your instruments, optimizing lab space. Adjustable shelves for easy LC stack customization with individual modules, enabling you to stack your instruments.|The InfinityLab Benchtop is an LC bench that fits into your existing bench setup. It is engineered to provide an adjustable and safe way to save lab space and to configure your LC system.|To compliment your HPLC bench, Agilent offer two shallow drawers for storing tools with your HPLC instrument and a small table offering space for columns during column exchange or the sample tray before samples are placed into the autosampler.
InfinityLab Assist, Level Sensing, and Sample ID Reader the new Infinity III HPLC and UHPLC Series technology.
The Agilent Infinity III HPLC and UHPLC systems provide scientists with advanced technology to stay connected and automate daily HPLC routines.|The InfinityLab Assist simplifies HPLC routine tasks, reducing operator time and minimizing errors. Automated HPLC workflows, real-time dashboard information, and assisted troubleshooting ensure seamless operations. Accessible onboard or remotely, users can shift their focus to enhancing HPLC results over manual tasks.|InfinityLab Level Sensing accurately monitors solvent levels, ensuring optimal productivity while preventing running dry. The Sample ID Reader automates sample identification, boosting efficiency and reducing mix-ups.|The Infinity III LC Series technology helps scientists maximize HPLC analysis efficiency, prevent errors, save costs, and stay connected in the chromatography lab. InfinityLab Assist, Level Sensing, and Sample ID Reader offer essential support and connectivity for modern HPLC labs, also available for Infinity II systems.
All the parts required to connect solvent bottles to your HPLC instrument
Agilent bottle head assemblies contain all the parts that are required to connect solvent bottles to your HPLC instrument. Each assembly contains a solvent inlet filter, tubing, a bottle cap, and fittings.
A range of high-quality HPLC tubing made from PEEK, PTFE, and other materials for connections in LC systems
Agilent provides a range of high-quality HPLC tubing made from PEEK, PTFE, and other materials to make connections in the low-pressure part of LC flow path. All Agilent HPLC tubes are manufactured to the highest quality standards to protect the integrity of your results at every step of your LC flow path. |Agilent color-coded PEEK tubing provide easy identification of internal tubing diameters to ensure optimal performance from low-flow to high-flow applications. The smooth internal surfaces of these HPLC PEEK tubing minimizes turbulences for improved resolution. HPLC PEEK tubing are flexible and easy to cut to length and can be used with PEEK and stainless-steel fittings. The excellent pH and solvent compatibility make them an ideal solution for standard HPLC applications up to 250 bar.|PTFE, FEP or PP HPLC tubes are widely used for non-pressure solvent and waste lines in larger internal diameters to connect solvent bottles to the HPLC pump and the outlet lines to the waste canisters.
Community Search Results
Liquid Chromatography
Liquid Chromatography
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Welcome to the Liquid chromatography Wiki ! You can find variety of Articles, whitepapers, LC best practices, LC troubleshooting guide, informative posters to hang on the walls of your lab, catalogs, videos and also the operator manuals for Agilent Liquid Chromatography products Access the content of your interest from the Agilent LC experts in the Wiki.
HI All, we have 1260 liquid chromatography. we ran the calibration stds. someone can help us to set up the method for calculation, because we know the concentration of total compounds but not the individual compound. how i can set up
HI All, we have 1260 liquid chromatography. we ran the calibration stds. someone can help us to set up the method for calculation, because we know the concentration of total compounds but not the individual compound. how i can set up
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Infinity 1260 Isoalhpa acid total amount known to us but we don't know the concentration of isoalpha acid 1 isoaplha acid2 and isoalpha acid 3 how i can set up the cal curve calculation
HPLC - What Happens in Reversed-Phase HPLC?
HPLC - What Happens in Reversed-Phase HPLC?
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Which Parameters Influence HPLC Separations? Eric de Witte, Norbert Reuter*, Global Technical Support for CSD, Middelburg, The Netherlands Introduction In liquid chromatography, the reversed-phase (RP) mode is everywhere. Even with a growing number of hydrophilic interaction chromatography (HILIC) applications, RP HPLC is the most common technique. This is a summary of what happens when the column parameters are changed. Increase Chain Length RP phases carry different organic ligands, starting from C1, C2, C3, C4, C6 and C8, to C18 or even longer.
Collection of LC (HPLC) Resources
Collection of LC (HPLC) Resources
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Here is a collection of Agilent LC Resources. Links to user manuals, firmware resources and maintenance videos , application resources , Agilent InterAct! (Virtual LC Simulation) , HPLC Advisor App , LC Supplies and Selector Tools and LC Troubleshooting Resources .
1100/1200 Infinity HPLC Autosampler Needles & CrossLab HPLC Syringes
Agilent offers a wide selection of CrossLab HPLC syringes to ensure accurate and effective sampling for HPLC analysis. Our HPLC autosampler syringes are compatible with competing legacy OEM autosamplers including the CTC PAL, Waters 717 & WISP ALS, Dionex WPS-3000 & ACC-3000, & Shimadzu SIL-10A/AF/10Ai. |Agilent also offer replacement HPLC needles & needle seat capillaries for all Agilent Infinity II autosamplers configurations & legacy HPLC autosamplers, including the original 1100 Infinity & HP G1811A XY ALS. Specific designs are available to achieve the best performance in any application area. HPLC needles should be routinely evaluated and replaced whenever worn or damaged to ensure complete sample transfer. Detailed maintenance and installation instructions are available on the Agilent Knowledge Portal.
High-quality HPLC capillaries in a large variety of materials and dimensions
HPLC capillaries are used in the high-pressure part flow path of LC flow path to make connections between different modules and column. All Agilent HPLC capillary tubing is engineered and manufactured to provide the best fit to instruments and columns to protect the integrity of your results at every step of your LC flow path. Agilent’s unique laser welding process ensures that all HPLC capillaries' ends are absolutely flat, eliminating any chance of capillary-induced dead volume.|Agilent offers HPLC capillaries in a variety of materials to suit your needs. Stainless steel capillaries with high flexibility and durability are ideal for most standard applications. HPLC capillaries made from MP35N, PEEK-lined stainless steel and titanium are inert and corrosion resistant, suited for bio applications.|All HPLC capillary tubing are color-coded to help you quickly identify the capillary id. Find the right HPLC capillaries quickly and easily using our online selection tool in the Tools section below.
Apply upgrade kits to optimize the performance of your HPLC pump and improve reliability of results.
Our pump upgrades are tailored to enhance the functionality and efficiency of your HPLC pump. Enjoy enhanced convenience for your day-to-day tasks with your HPLC and UHPLC instrument to improve reliability of your results. |Certain upgrades can be easily user-installed and integrated seamlessly into your existing HPLC pump. Furthermore, our team of experts is always available to provide support and guidance throughout the upgrade process. Whether you have questions about compatibility or need assistance with installation, we are here to help you every step of the way.
HPLC columns for the analysis of per- and polyfluorinated compounds (PFC or PFAS).
The InfinityLab PFC Delay Column was designed and tested to efficiently delay elution of per- and polyfluorinated compounds (PFC or PFAS) released from pump parts, solvent lines and solvents that would interfere with detection and quantification of PFC in samples, including drinking water, soil, and food. This product is recommended to be used with the InfinityLab PFC-free LC conversion Kit (5004-0006). The InfinityLab PFAS Delay Column is not an analytical column and is installed in the pump (see technical note shipped with the kit or literature below).
HPLC solvent inlet filters for solvent filtration in flow path
HPLC solvent inlet filters represent the first barrier for retaining particles, precipitates, and microbes from mobile phases, buffers, and salt solutions at HPLC inlet. Agilent provides a wide range of high-quality glass, PEEK, and stainless steel HPLC inlet filters with different pore sizes. |Agilent solvent inlet filters are designed for and tested on Agilent instruments to ensure best fit and protection of your valuable columns against particle clogging. These inlet solvent filters are critical for preventing pressure increases and contamination, leading to extended column lifetime, increased analytical efficiency and decreased cost per sample. |Further down the flow path, Agilent inline filters can be installed at the high-pressure side to filtrate even smaller particles from solvents, samples and system worn parts.
Color-Coded HPLC Manual Syringes & Replacement Liquid Chromatography Needles.
Agilent CrossLab HPLC manual syringes feature a blunted tip for direct HPLC injections as well as sample pipetting. The blunt tip has chamfered and polished edges to prevent damage to the LC injection valve’s rotor seal and stator face. Our HPLC manual syringes also have metallic back-flange with flattened sides, which can prevent syringes from rolling off the bench. These manual microsyringes are distinct and easy-to-read with graduations made clearly visible by a colored backing strip, differentiated by volume (0.5µL to 50 mL). |Together with HPLC manual syringes, Agilent also offers compatible HPLC needles. Our liquid chromatography needles are electropolished to ease injection and retraction force on the rotor valve, preserving the seals lifetime.|Each microsyringe comes with a Certificate of Conformance and eco-friendly packaging that doubles as a syringe stand. For more help choosing the right syringe for your application, check out our syringe selection tool below.
HPLC bottles & waste containers designed to provide optimal performance for your LC applications
Agilent offers InfinityLab HPLC bottles as mobile phase reservoirs and HPLC waste containers with various capacities and thread sizes for waste management.|The ergonomic design of the InfinityLab HPLC glass bottles ensures easier use and safer handling, reducing the risk of accidents in the lab. The 1 and 2 liter HPLC mobile phase bottles are available in clear and amber glass, suitable for situations where sample visibility is important. The 1 liter bio solvent bottle is a polypropylene alternative to glass bottles, best for sodium-sensitive MS applications.|All InfinityLab solvent bottles have a GL45 thread that works best with the InfinityLab Stay Safe caps to prevent evaporation of solvent vapors. This range ensures optimal compatibility with your InfinityLab LC Series instruments, enabling seamless incorporation into your LC workflow. The InfinityLab HPLC waste containers are available in capacities ranging from 5 to 10 liters and are compatible with Stay Safe caps for waste canisters, which include charcoal filters with time strips.
HPLC Solvent Filters to improve LC system performance by removing contaminants and degassing the HPLC solvents.
HPLC solvent filters, also known as mobile phase filters, are used to filter mobile phase buffers as a daily routine part of HPLC sample analysis. Agilent offers solvent filtration assembly and HPLC filter membranes as HPLC solvent filters to degas and remove particle contaminants from HPLC solvents and butters. |The Agilent InfinityLab solvent filtration assembly is manufactured from high-quality borosilicate glass components and can hold a 47 mm HPLC filter membrane to remove particulates from liquid media. Using our mobile phase filters prior to your analysis decreases piston wear from particulates, prevents column and capillary clogging, increases pump seal lifetime, and minimizes background noise in HPLC chromatograms. |Solvent filtration is especially important in Biopharma applications. Water-based buffer solutions, predominant with Biopharma applications, should be filtered daily to avoid microbial growth in your solvent bottles.
High-quality HPLC unions and adapters for a range of equipment and applications
HPLC unions and adapters are connectors that connect the same or different types of fitting threads or ports. Agilent offers a wide variety of high-quality HPLC unions to suit different instruments and connection types. |The zero-dead-volume (ZDV) HPLC stainless steel unions with and without fittings are typically used for standard HPLC. The ZDV unions (HPLC PEEK union or HPLC stainless steel union with PEEK inserts) are suited for bio applications. The high-flow unions (no fittings) are developed for use with preparative HPLC. A range of adapters and connectors are also available, including barbed Y-connectors, T-connectors, and female-to-female or male-to-female adapters.
Enhance the functionality of your HPLC multisampler or vialsampler by applying various upgrade kits.
To improve the functionality of your HPLC multisampler or vialsampler, you can apply a range of upgrade kits that will improve the sampler performance and provide additional features. These injector upgrades can streamline your workflow and lead to more accurate and reliable HPLC results in your day-to-day analysis.|Whether it’s the sample thermostat, dual-needle, multidraw, or multiwash technology, by upgrading your HPLC injector you can unlock a whole new level of efficiency and convenience. Certain upgrades can be easily user-installed and integrated seamlessly into your existing HPLC pump.
Expand the functionality of your LC system with various HPLC upgrades and accessories
To expand the functionality of your Infinity II components and accessories, Agilent offers various HPLC upgrades and accessories to enhance your 1260 or 1290 Infinity II. Most parts can be readily installed by the user.
HPLC Fittings and ferrules made of different materials to ensure reliable sealing and optimal performance in LC
Agilent offers a large variety of HPLC fittings to enable connections of your HPLC capillaries onto instruments, valves or columns, for both Swagelok-type and metric M4-type connections. The HPLC ferrule is the primary sealing component of the fitting assembly. Agilent offers stainless steel ferrules, PEEK ferrules, and HPLC ferrules made from other different materials for fixed or removable fittings. |Utilizing the correct chromatography fitting style and format is important for ensuring accuracy and robust performance in every aspect of your apparatus. |InfinityLab Quick Connect HPLC fittings offer truly finger-tight connections up to 1300 bar, for column connections as easy as pressing a lever.
For harder-to-reach areas of the LC instrument, try InfinityLab Quick Turn HPLC fittings that are finger-tight up to 400 bar and can reach 800 bar by using a mounting tool or 1300 bar using a wrench.
Swagelok fittings made from stainless steel provide high pressure permanent sealing, suited for positions that don’t need to be reconnected often.|PEEK fittings or polyketone fittings are finger-tight up to 400 bar and can be used in low-pressure area in the flow path. |Our range of HPLC fittings can ensure the highest performance of various LC systems and applications.
For harder-to-reach areas of the LC instrument, try InfinityLab Quick Turn HPLC fittings that are finger-tight up to 400 bar and can reach 800 bar by using a mounting tool or 1300 bar using a wrench.
Swagelok fittings made from stainless steel provide high pressure permanent sealing, suited for positions that don’t need to be reconnected often.|PEEK fittings or polyketone fittings are finger-tight up to 400 bar and can be used in low-pressure area in the flow path. |Our range of HPLC fittings can ensure the highest performance of various LC systems and applications.
Increase column thermostat flexibility with various upgrade kits for convenient column switching.
Our upgrades for the multicolumn thermostat feature various valve heads for seamless column switching, saving you time and effort. You will be able to easily switch between different column chemistries or dimensions without the need for manual column changes. It improves productivity and reduces column damage risk during the switching process. |Say goodbye to tedious manual column changes and streamline your workflow with our column compartment upgrades. With Agilent column oven upgrades, you can be confident in the quality and reliability of our products. We strive to provide you with the best solutions to optimize your HPLC system and meet your analytical needs.
Get more information from your analysis by using additional HPLC software components
With HPLC software upgrades and add-ons, you can enhance the capabilities of your analysis and extract even more valuable information. These upgrades provide seamless integration with your existing HPLC system, allowing you to streamline your workflow and improve productivity. |The Lab Advisor Advanced Software offers enhanced instrument control capabilities. This means you can easily monitor and analyze various parameters of your HPLC and UHPLC systems directly within the software interface. This level of control not only simplifies operation but also ensures reproducibility and consistency in your analyses. Explore new components and add-ons available for your Chromatography Data System Software and unlock the full potential of your HPLC analysis.
FLD detector with UV-like simplicity— confidence at the limit, at UHPLC speed
The Agilent 1290 Infinity III Fluorescence Detector (FLD) defines precision and simplicity in liquid chromatography fluorescence detection. Delivering exceptional sensitivity and speed for ultrahigh-performance liquid chromatography (UHPLC) workflows, this FLD detector reveals ultra-trace analytes with outstanding clarity. Its advanced optical design enables rapid excitation and emission wavelength switching, low-dispersion flow cells, and intuitive operation. |Providing spectral data in both excitation and emission dimensions, this fluorescence detector ensures accurate characterization of complex samples across biopharma, food safety, and environmental UHPLC applications.