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1260 Infinity III Prime Bio LC System

A biocompatible, quaternary LC system with a pressure range up to 800 bar and flow rates up to 5 mL/min

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The 1260 Infinity III Prime Bio LC System is a versatile HPLC instrument for bioseparations and offers outstanding functionality and operational convenience for biochromatography, and entry-level quaternary bio UHPLC at pressures up to 800 bar and flow rates up to 5 mL/min.|The 1260 Infinity III Prime Bio LC System, consisting of biocompatible material for use in biopharma (e.g., critical quality attributes) and other applications utilizing high-salt and extreme-pH conditions, ensures the integrity of your biomolecules and robustness of the system.

1260 Infinity II Preparative Valve-Based Fraction Collector

High-volume fraction collection for purification workflows up to 200 mL/min.

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The 1260 Infinity II Preparative Valve-Based Fraction Collector is a compact, full-featured fraction collector for daily compound isolation and purification by LC. Optimized for collection of up to 11 high-volume fractions its versatile design allows operation in both routine sample purification workflows and recovery applications. The built-in fraction delay sensor allows precise and automatic calibration of the fraction collector for accurate sample collection.|This fraction collector accepts multiple detectors as trigger sources and includes all standard collection modes like time-based and peak-based. It can be used on any InfinityLab Purification System and its small dimensions occupy virtually no additonal space on the instrument. Blow-outs of nitrogen or compressed air purge the outlet lines after completed collection and help minimize carryover.

1260 Infinity III Multisampler

Injection from vials and micoplates of up to 6,144 samples at up to 800 bar.

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The 1260 Infinity III Multisampler is a multipurpose autosampler that handles vials and microplates. Injecting at pressures up to 600 or 800 bar, the Multisampler is a compact module with a capacity of up to 6,144 samples, all within the footprint of an Agilent LC stack.|The 1260 Infinity III Multisampler is optimized for highest flexibility. The unique dual-needle design enables cycle times to be reduced by overlapping sample runs and injection cycles. The multiwash capability reduces carryover to less than 9 ppm.

1260 Infinity II Preparative Manual Injector

Easy and reliable injection of up to 20 mL of sample for routine compound purification.

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The 1260 Infinity II Manual Preparative Injector is ideal for routine purification of milligram and multigram quantities at flows up to 200 mL/min. Modular sample loops in various sizes up to 20 mL volume allow application in many different purification workflows.|This injector is the entry into purification workflows, easy to use, and highly reliable. Attaching to the Agilent 1260 Infinity II Preparative Column Organizer, it does not add to the total instrument bench space. This affordable, hand-operated injection valve fits to any InfinityLab Purification System.

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1260 Infinity II Bio-SEC Multidetector System

Characterizes proteins and biomolecules accurately and reproducibly with light scattering detection.

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The 1260 Infinity II Multidetector Bio-SEC System comprises light scattering and dynamic light scattering to allow determination of protein shape and size without the need for SEC column calibration. Size exclusion chromatography (SEC) is the standard method to determine and quantitate monomers, dimers, aggregates, and potential degradants and is a common requirement for regulatory approval. The detector has a simple low dead volume interface to the 1260 Infinity III Bio-inert LC.

1260 Infinity III Online Sample Manager

Direct injections and retain-sample functionality for fast, confident process monitoring.

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The 1260 Infinity III Online Sample Manager is an online sampling module that connects the analytical world with the process world. The module provides automated sample analysis via direct injections or retained samples from flow reactors, batch reactors, as well as upstream bioreactors and downstream purification devices. |The Online Sample Manager supports both classical flow-through injection and Agilent Feed Injection, mediating the chromatographic sample diluent incompatibility of challenging process samples. The Online Sample Manager provides automated dilutions of up to 1:1000, retain-sample functionality, and direct analysis of the process samples, as well as automated sample preparation.

1260 Infinity II Multi-Angle Light Scattering Detector

MALS Detector for determination of absolute molecular weight in GPC/SEC.

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Light scattering (LS) in GPC/SEC applications is one of the few absolute techniques for the characterization of macromolecules. The 1260 Infinity II Multi-Angle Light Scattering Detector (MALS Detector) provides accurate determination of absolute molecular weight, size, radius of gyration, and conformation information of macromolecules in solution. |The 20-angle static light scattering detector allows for the simultaneous detection of the time-averaged scattering intensity at 20 different detection angles. A cylindrical flow cell design ensures robust and reliable operation for accurate results in GPC/SEC systems and applications.

1260 Infinity III Variable Wavelength Detector

High-sensitivity, single-wavelength UV detection from 190 to 600 nm at 120 Hz and ±2.5 µAU/cm noise.

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The 1260 Infinity III Variable Wavelength Detector enables highest productivity with fast analysis at up to 120 Hz data rates. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.| Lowest baseline noise and drift results in lowest detection limits for robust quantification of trace level components.

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1260 Infinity II Bio-SEC System

Reliable analysis of biological polymers with metal-free flow path and optional advanced detectors

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The 1260 Infinity II Bio-SEC System is a size exclusion chromatography (SEC) system designed to meet the challenges of analyzing biological polymers. The metal-free flow path ensures robust and reliable operation for harsh analytical conditions at high salt concentrations and extreme pH values. The 1260 Infinity II GPC/SEC Column Thermostat with optional cooling ensures sample integrity. And advanced detection methods, like multi-angle light scattering with SEC MALS detection, allow for the measurement of absolute molecular weight, size, and conformation of macromolecules in solution.|This unique combination makes the system the perfect solution for protein molar mass, purity, and aggregation analysis with advanced detection.

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1260 Infinity III Prime Online LC System

Fast, automated, and robust process monitoring via LC applications.

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The 1260 Infinity III Prime Online LC System is a process analysis system, enabling automated process monitoring and providing real-time data for greater control and accurate monitoring of critical quality attributes. Supporting the needs of scientists using process analytical technology in the pharmaceutical industry, this real-time monitoring LC instrument supports direct injections for fast process monitoring.|This online LC process analysis system also features retain-sample functionality for dilutions or automated sample preparation steps, multimethod analysis, or sample archiving purposes for offline quality control.

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1260 Infinity III Prime LC System

HPLC/UHPLC quaternary solvent mixing with pressure range up to 800 bar and flow rates up to 5 mL/min.

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The 1260 Infinity III Prime LC System is an analytical ultrahigh-performance liquid chromatography instrument. It operates at high pressures and can handle flow rates up to 5 mL/min for using UHPLC columns packed with sub-2 μm particles. This HPLC/UHPLC system gives you the utmost confidence in your HPLC results and is a perfect front end for your LC/MS system. A broad range of detection options is available on this quaternary UHPLC system for routine HPLC analysis. |As a quaternary system, this flexible HPLC/UHPLC instrument combines high performance and high flexibility. It has gradient formation and mixing performance equivalent to a binary-like system. It provides quaternary and ternary solvent mixing for excellent retention time reproducibility, accuracy, and precision. |This system also offers technology functions, such as Agilent Intelligent System Emulation Technology (ISET) and Blend Assist. With ISET, you can seamlessly transfer methods from Agilent or non-Agilent HPLC/UHPLC systems, eliminating the need for time-consuming method redevelopment. Blend Assist simplifies UHPLC system workflows and accelerates method development by automating mobile phase blending.

1260 Infinity III Bio-Inert Pump

Quaternary solvent delivery for biological and extreme pH applications up to 600 bar.

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The 1260 Infinity III Bio-Inert Pump is a quaternary UHPLC pump that is made of bio-inert material for use in biological and extreme pH applications. This pump enables gradient formation and solvent blending from up to four solvents and operates at pressures up to 600 bar and flow rates up to 10 mL/min.|The 1260 Infinity III Bio-Inert Pump is resistant to corrosion by high salt concentrations (2 M) and can handle a wide pH range from 1–13, and even up to pH 14 for short-term use. This pump is ideal for protein and NBE characterization.

1260 Infinity III Binary Pump

Binary solvent delivery at pressures up to 600 bar and flow rates up to 5 mL/min.

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The 1260 Infinity III Binary Pump uses high-pressure mixing for UHPLC gradient formation and solvent blending from one or two solvents at pressures up to 600 bar and flow rates up to 5 mL/min.|The 1260 Infinity III Binary Pump is ideally suited for a broad range of UHPLC applications using columns with conventional, superficially porous or sub-2-micron particles. This pump delivers reproducible gradients for routine HPLC or UHPLC applications with UV or MS detection where speed, resolution, and uncompromised data quality are essential.

1260 Infinity III SFC Binary Pump

600 bar and 5 mL/min power range for extended SFC and SFC/MS application reach.

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The 1260 Infinity III SFC Binary Pump is a supercritical fluid chromatography (SFC) pump that supports high-pressure gradient formation and precise mobile phase delivery up to 5 mL/min and 600 bar. It can be used with a broad range of chiral and achiral columns, and ideal for fast, high-throughput SFC and SFC/MS applications.|The 1260 Infinity III SFC Binary Pump is equipped with special pistons and seals for utmost robustness under SFC conditions. Carbon dioxide (CO₂) is delivered through channel A while organic modifier can be added through channel B for either isocratic or gradient elution. Channel B is fitted with a purge valve to facilitate rapid change of organic modifier.

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1260 Infinity III Amino Acid Analysis System

Automated amino acid analysis with latest HPLC technology and proven precolumn derivatization.

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The 1260 Infinity III Amino Acid Analysis System provides a complete solution for the automated HPLC amino acid analysis. It combines the latest developments in HPLC instrumentation and column technology with proven precolumn derivatization chemistry for fast and sensitive amino acid analysis.|Achieve the next level of productivity by using the Agilent 1260 Infinity III Vialsampler for automated precolumn derivatization. Ready-to-use reagents and standards, combined with the application support from Agilent, makes this solution the perfect tool for automated amino acid analysis in the food and pharmaceutical industry.

1260 Infinity III Fluorescence Detector Spectra

High-sensitivity, multisignal data and spectra in a single run at up to 148 Hz.

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The 1260 Infinity III Fluorescence Detector Spectra is a sensitive HPLC fluorescence detector that simultaneously provides quantitative data and fluorescence spectra from a single run, enabling rapid method optimization for HPLC FLD Detector Spectra experiments.|Use the online spectral information for rapid method optimization and verification of separation quality. High-speed detection features up to 148 Hz data rates for very narrow peaks. This detector can be used with any Agilent InfinityLab LC Series system or other LC instrument.

1260 Infinity III SFC Multisampler

High capacity with LC-like injection precision and reproducibility for SFC and SFC/MS applications.

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The 1260 Infinity III SFC Multisampler is a high-capacity autosampler for supercritical fluid chromatography, bringing LC-like injection precision and reproducibility to SFC. The wide injection range supports flexible injection volumes from 0.1 to 90 µL without changing sample loops.|The 1260 Infinity III SFC Multisampler is compatible with 2 mL vials and well plates for fast, high-throughput (up to 6,144 samples) SFC and SFC/MS applications. Innovative Feed Injection technology pressurizes the sample prior to injection into the carbon dioxide (CO₂) stream for utmost injection precision and reproducibility.

1260 Infinity III Diode Array Detector WR

Wide-range, UV-spectral detection from 190 to 950 nm with 120 Hz sampling.

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The 1260 Infinity III Diode Array Detector WR offers multiple wavelength and full spectral detection up to a 120 Hz sampling rate. Maximum flexibility is guaranteed with a wide wavelength range of 190 to 950 nm with dual-lamp design, as well as the choice of 15 analytical, preparative, and SFC flow cells. Even at high data rates, the 1260 Infinity III DAD WR enables precise identification, quantification, and peak purity analysis at trace levels.

1260 Infinity III Bio-Inert Multisampler

High capacity up to 6,144 samples and 600 bar, and metal-free flow path for bio-applications.

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The 1260 Infinity III Bio-Inert Multisampler injects samples from microplate and vial at pressures up to 600 bar. It is a compact module offering a capacity of up to 6,144 samples. With its multiwash capability, you can reduce carryover to less than 9 ppm.|The metal-free sample flow path makes it the ideal injector for all biorelated applications, including analysis of mAbs, proteins in general, and oligonucleotides. For temperature-sensitive samples, a highly efficient compressor-based thermostatting system can be added. The ceramic needle, PEEK needle seat, and stainless steel-clad PEEK capillaries ensure highest injection accuracy and precision.

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1260 Infinity III Bio-Inert Analytical-Scale LC Purification System

Bio-inert analytical HPLC purification system with corrosion-resistant flow path to protect biomolecules

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The 1260 Infinity III Bio-Inert Analytical-Scale LC Purification System is a dedicated solution for purifying large biomolecules in biological samples, including monoclonal antibodies and proteins. |This bio analytical preparative HPLC system comes with metal-free components in the sample flow path. The absence of iron and steel in solvent delivery lines ensure the integrity of your biomolecules, minimize unwanted surface interactions, and increase column lifetime.|The power range of this bio-inert HPLC purification system expands from low pressure, for traditional biopurification columns, up to high pressure, for sub-2-micron analytical biocolumns. The 1260 Infinity III Bio-Inert Fraction Collector offers a cooling module to prevent degradation of thermally labile biomolecules. Buffer Advisor software facilitates faster pH scouting and easy buffer/solvent preparation for ion-exchange chromatography (IEX).