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A Practical Guide for 3D Cell Culture Systems and Optimizing Spheroid Imaging Assay - Part 1 of 2

3D cell culture systems have become a well-established in vitro experimental approach to model human disease for a broad range of research applications.

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FAQs
Imaging & Microscopy - FAQs

Commonly asked questions and answers about Agilent BioTek cell imaging multimode readers, automated microscopes and software for imaging & microscopy.

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Article Reprints
Improving T-Cell Activation and Serial Killing Through Metabolic Modulation (PDF)

This editorial showcases Agilent solutions for metabolic modulation enhancing CAR T-cell activation, persistence, and serial killing for improved cancer immunotherapy

  • Publication Part Number: 5994-7739EN
  • Created: 04 Nov 2024
  • 830 KB
  • PDF
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FAQs
Microplate Readers - FAQs

Commonly asked questions and answers about Agilent BioTek microplate readers, including hybrid multimode readers, multimode readers, and absorbance readers.

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Cell Imaging Multimode Readers

Agilent BioTek Cytation cell imaging multimode readers combine automated microscopy and conventional microplate detection in a modular, upgradable platform. Modularity offers the ability to configure with the required microscopy and detection modes...

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Microplate Imagers

Agilent BioTek automated imagers and cell imaging multimode readers deliver versatility, performance, and value, while bringing your science to life. Gen5 software drives the automated process known as Augmented Microscopy to help capture...

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Cell Imaging & Microscopy Software

Cell imaging and microscopy software offers automated control and analysis of image acquisition and processing. From whole organism imaging to high magnification subcellular image analysis, Agilent BioTek Gen5 helps transform raw data and images...

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Imaging Objectives

High quality Olympus and Zeiss objectives from 1.25x to 100x air and oil immersion

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High-quality Olympus and Zeiss objectives to suit a wide range of applications. Choose objectives from 1.25x to 60x air for Agilent BioTek Cytation cell imaging multimode readers and up to 100x air and oil immersion for Agilent BioTek Lionheart automated microscopes. Standard and phase contrast objectives are also offered.
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BioTek AutoScratch Accessory & Supplies

AutoScratch and Scratch Assay app for automated, kinetic cell migration and scratch wound assays

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BioTek AutoScratch wound making tool automatically creates reproducible scratch wounds in cell monolayers grown in 24- or 96-well microplates for cell migration and invasion studies. AutoScratch automates the sample prep for imaging using BioTek Cytation cell imaging multimode readers and Lionheart automated microscopes.|The Scratch Assay App automates image collection and analysis of wound width, wound confluence and maximum wound healing rate.

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BioTek Cytation Hybrid Multimode Reader

Cytation Hybrid cell imaging multimode readers with Hybrid Technology for sensitivity and flexibility

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The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. The proprietary Hybrid Technology delivers the sensitivity of filters and the flexibility of monochromators.|Detection modes available include UV-Vis absorbance, fluorescence, and luminescence. Fluorescence microscopy to 60x can be added to expand the range of applications to fixed and live-cell imaging. The included Agilent BioTek Gen5 is designed for uncomplicated data collection, processing, and analysis for the most complex assays.
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Peltier Cooling Module

The Peltier cooling module for temperature sensitive assays and environmental stability in Cytation

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The Peltier cooling module promotes a rapid interior cool down after incubated processes. The rapid cooling enables efficient switching between multiple applications without unwanted temperature influences.|For temperature sensitive assays typically run at ambient, or room temperature, the cooling module maintains environmental stability within the Agilent BioTek Cytation instrument. This stability allows < 1 °C rise in ambient temperature, regardless of external and internal temperature fluctuation. Rigorous temperature control gives you confidence in your assay results.
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Imaging Stage Control Joystick

Joystick controller for BioTek Cytation or Lionheart imager

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The joystick controller enables easy, precise maneuvering in and around your sample when used with an Agilent BioTek Lionheart or Cytation imagers.
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Imaging Objective Accessories

Accessories to optimize objectives in Agilent BioTek imagers

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Objective accessories include spare alignment jigs and other tools to optimize our range of high quality standard, phase and oil immersion objectives for Lionheart and Cytation imagers.

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BioTek BioSpa Live Cell Analysis System

BioSpa live cell analysis system with multiple imaging modes for a wide range of microscopy applications

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The BioTek BioSpa live cell analysis system offers unique benefits for a wide variety of live cell imaging applications in up to 8 microplates or other labware. With 5 imaging modes, the system enables a wide range of microscopy applications.|Capture detailed cellular images, get cell-level analyses for migration and wound healing assays, 3D spheroid formation, and many other kinetic applications. Built-in scheduling, environmental monitoring, and available liquid handling for walk away automation. Multiple users can run simultaneous processes for maximum efficiency in the lab.
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Imaging Filter Cubes

Imaging filter cubes for BioTek Cytation and Lionheart microscopes and imagers

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Imaging filter cubes for confocal and widefield imaging are available. Widefield cubes are available in more than 20 color filter cubes to pair with LED cubes. This extensive offering of filter cubes covers a broad range of fluorescent dyes for many applications.
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BioTek Take3 & Take3 Trio Microvolume Plates

Microvolume plates are compatible with the Cytation, Synergy, and Epoch readers

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The Take3 and Take3 Trio microvolume plates increase the functionality of your microplate reader by decreasing the required sample size down to a 2 μL volume. Each plate is compatible with the Cytation, Synergy, and Epoch readers for microvolume absorbance and fluorescence quantification.|Microvolume quantification is fast and easy by measuring multiple 2 µL samples at the same time, without diluting and without the need for specialized equipment. Preprogrammed nucleic acid and protein protocols in the Take3 app bring confidence in your quantification data.
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Dichroic Mirrors

Dichroic mirrors for excitation (EX) and emission (EM) available in full- and half-size

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Dichroic mirrors are offered for excitation (EX) and emission (EM), and are available in full-size and half-size. Agilent BioTek Synergy and Cytation multimode reader filter cubes can contain up to two EX and EM filter pairs and can fit up to two half-size mirrors or one full-size mirror.
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Applications
Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (PDF)

This study demonstrates the use of a novel, automated tool to create scratch wounds in cell monolayers formed on the bottom of a microplate.

  • Publication Part Number: 5994-2585EN
  • Created: 01 Apr 2021
  • 1 MB
  • PDF
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Applications
Histology and Slide Scanning for Light Microscopy (PDF)

Several colorimetric and fluorescent assays are described incorporating automated image acquisition and analyses using Agilent’s high-content imaging instruments.

  • Publication Part Number: 5994-3068EN
  • Created: 01 Feb 2021
  • 9 MB
  • PDF
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Applications
High-Throughput Methods to Quantitatively Evaluate TGF-β Signaling in Epithelial-to-Mesenchymal Transition (PDF)

TGF-β/SMAD signaling is quantitatively evaluated in biochemical, cellular, and spheroid-based assays using a cancer model of epithelial-to-mesenchymal transition.

  • Publication Part Number: 5994-6709EN
  • Created: 22 May 2025
  • 4 MB
  • PDF

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