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This application note highlights the wide range in instrumentation, plastics, and reagents that Agilent offers to enable a complete workflow for cell-based toxicity assays.
This application note demonstrates an automated γH2AX assay workflow using a novel cell imaging multimode reader with advanced data analysis capabilities.
The goal of this application note is to demonstrate how in situ fluorescence hybridization experiments can be successfully imaged and analyzed using the Cytation.
This application note demonstrates a novel 3D wound healing assay model that can overcome 2D assay limitations.
This study demonstrates the ability to monitor the effect of histone methylase and demethylase inhibitors that selectively induce apoptosis in cancer cell lines.
Collection of Cell Analysis Resources
Collection of Cell Analysis Resources
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Changes In Metabolic Phenotype And Cellular ATP Production During CD4+ T Cell Activation Characterization of fuel dependencies in multidrug resistant breast cancer cells A Novel Solution to Better Understand the Link Between T Cell Metabolism and Function Quality Assessment of Metabolic Fitness during T Cell and NK Cell Expansion Determining Mechanisms of Mitochondrial Toxicity using Agilent Seahorse XF Technology Determining the Metabolic Consequences of Pharmacological Modulation of T Cell Activation Metabolic discovery platform:“Seahorse-Bravo” Screening and validation of mitochondrial inhibitors and uncouplers using a novel toxicity index derived from mitochondrial oxygen consumption rates Cell-based screening of drug-induced metabolic dysfunctionHigh-throughput in vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation High-throughput in vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation Multiplexed Screening of Drug-Induced Mitochondrial Dysfunction and Cytotoxicity Simple High-Throughput Workflows for Assessing Drug-Induced Mitochondrial Dysfunction Using Oxygen Consumption Measurements Application Notes Application Notes (overall link) A Customized XF Workflow for Detection and Characterization of Mitochondrial Toxicity Assessing functional metabolism using cells sourced from blood Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay Assessing mitochondrial dysfunction in primary cardiomyocytes Assessing the Mitochondrial Toxicity of Environmental Pollutants Using the Agilent Seahorse XF Extracellular Flux Analyzer Assessing NK Cell Bioenergetic Metabolism Using Extracellular Flux Analysis Assessing T Cell Bioenergetic Poise and Spare Respiratory Capacity Using Extracellular Flux Analysis Assessing the impact of autophagy on cellular metabolism Assessing therapies for traumatic brain or spinal cord injuries Bioenergetic analysis of primary neuronal deficiencies Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes Cancer Cells Evoke Flexibility By Maintaining a Bioenergetic Balance Between Glycolysis and Mitochondrial Respiration Linked to Metastatic Potential Cell-Based Screening for Drug‑Induced Mitochondrial Toxicity Characterization of Glycolysis with a Panel of Common Cellular Models Using Agilent Seahorse XF Technology Defining metabolic phenotypes in cancer cells Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs) Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets Evaluating changes in cell metabolism in neuroimmune and neuropsychiatric disorders Functional validation of Genomic and Proteomic data Gaining Insights into Disease Biology for Target Identification and Validation using Seahorse XF Technology Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia Maximizing Efficiency and Performance of Seahorse XF Assays with the Bravo Automated Liquid Handling Platform Measurement of Mitochondrial Toxicity Using Primary Hepatocytes Measuring bioenergetic profiles of human adipocytes Measuring functional metabolism of brite adipocytes Measuring glycolysis and oxidative metabolism in cancer cells Measuring glycolytic function in cells Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer Measuring the metabolic switch in cancer cells Metabolic Pathway Determination Using the Seahorse XF24 Analyzer Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5 Quantitative Analysis of Neutrophil Activation Using Agilent Seahorse XF Technology Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates Real Time Discrimination of Inflammatory Macrophage Activation Using Agilent Seahorse XF Technology Revealing Cellular Metabolic Phenotype and Function Using Agilent XF Substrate Oxidation Stress Tests Using the Agilent NovoCyte Flow Cytometer for Immune Suspension Cell Normalization in Agilent Seahorse XF Assays XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System Quick Reference Guides Quick Reference Guide: Calculating Proton Efflux Rate (PER) Data Agilent Seahorse XF Buffer Factor Protocol Quick Reference Guide Informative and Instructional Videos Cell Analysis Video Library (Overall) Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics How to accelerate your analysis workflow with Agilent Seahorse Analytics How to transform data into insight with Agilent Seahorse Analytics How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics Seahorse XF Mito Tox Assay with Seahorse Analytics Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software Analyze Agilent Seahorse XF Substrate Oxidation Stress Test data using Agilent Seahorse Analytics Bravo Automation-XF Assay Demonstration Bravo Automation-XF Assay Streamline Cell Analysis Normalization System Overview Cell Energy Pathways How to analyze XF neutrophil activation result data How to accelerate your analysis workflow with Agilent Seahorse Analytics How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics How to transform data into insight with Agilent Seahorse Analytics Measuring Cell Metabolism in Transient Microchamber Seahorse XF Mito Tox Assay with Seahorse Analytics Seahorse Analyzer Multi Port Drug Injection Seeding Cell Culture Microplates XF Real-Time ATP Rate Assay Webinars Webinars (overall link) Report Generators – Single and Multi-file (if available) Seahorse XF Cell Mito Stress Test Report Generators Seahorse XF Glycolytic Rate Assay Report Generators Seahorse XF Mito Fuel Flex Test Report Generator Seahorse XF Real-Time ATP Rate Assay Report Generators Soluble Sensors Overall: Plate Reader Compatibility MitoXpress Xtra Oxygen Consumption Assay Plate Reader compatibility chart Ph-Xtra Glycolysis Assay Plate Reader compatibility chart MitoXpress Intra Plate Reader Compatibility Guide Instrument Setup Guides – MitoXpress Xtra Oxygen Consumption Assay Setup Guides MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Perkin Elmer plate readers MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Tecan plate readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BMG Plate Readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for Molecular Devices Plate Readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BioTek Plate Readers Instrument Setup Guides – pH-Xtra Glycolysis Assay Setup Guides pH-Xtra Glycolysis Assay Instrument setup guide for Perkin Elmer plate readers pH-Xtra Glycolysis Assay Instrument setup guide for Tecan plate readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for BioTek Plate Readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for Molecular Devices Plate Readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for BMG Plate Readers User Manuals and Technical Overviews Agilent pH-Xtra Glycolysis Assay Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method) Agilent MitoXpress Intra Intracellular Oxygen Assay Agilent Mito-rOCR Assay Kit User Guide Mito-rOCR Gen5 and Demo Files Improved Assay Performance and Workflow with Agilent Mito-rOCR Compared to Oil-Based OCR Assays Application Notes and Posters Assessing the Impact of Drug Treatment on Cardiomyocyte Function Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay Metabolic Profiling of Cells in 3D Cultures using MitoXpress Xtra and pH-Xtra Assays Screening for Drug-Induced Mitochondrial Dysfunction Using MitoXpress Xtra on Isolated Mitochondria Multiplexed Assessment of Mitochondrial Function Combining the Agilent Mito-rOCR Assay with Live-Cell Imaging Multiplexed assessment of mitochondrial function with real-time oxygen consumption measurement and live cell image analysis How-To Videos How Mito-rOCR works Agilent Mito-rOCR Data Analysis Agilent Mito-rOCR Product Innovations Software Data Visualization Tool Agilent MitoXpress & pH-Xtra Data Visualization Tool User Manual Have a question? Ask the Cell Analysis community
Webinar Digest Update - 5/15/2025
Webinar Digest Update - 5/15/2025
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The Digest of upcoming webinars has been updated as of May 15. Register and attend Pathology A La Carte Webinars for CEU credit! Check out Basic Intro to NGS on May 27. Notable additions include: GC & GCMS (1) Check out: ASTM 8322: Elemental Analysis of Crude Oils and Residual Fuels Using MPAES on May 20 Virtual Bioanalyzer Upgrade Summit on June 23 USP 467 Residual Solvent: Application Updates Tips & Tricks on May 22 Rapid Vaccine Production and Testing Workflows at Boehringer Ingelheim Utilizing ViQi AVIA ™ AI technology and Agilent Cytation Brightfield Imaging on May 21 Mastering Automation in Dissolution Workflows on May 15 The Unsung Heroes of Analytical Chemistry: The Vital Role of Autosampler Vials on June 26 A Systematic Approach to GPC/SEC Method Development on May 22 Part 1: Characterizing Catalysts Using an UV-VIS/NIR System with the Praying Mantis Diffuse Reflectance Accessory on May 22 Basic Intro to NGS on May 27 Seminar 1 – OpenLab CDS – The Control Panel and Data Acquisition on June 3 Seminar 2 – OpenLab CDS – Data Analysis on June 10 Seminar 3 – OpenLab CDS – Custom Calculator and Reporting on June 17 Seminar 4 - OpenLab for MS Data on June 24 What is new with ICP-MS Mass Hunter 5.3? Optimize your Method Data Analysis for a complete evaluation of the lab analysis on June 12 See the complete list here: Webinar Notifications A listing of past recorded webinars is available here: Agilent Recorded Webinars Thank you.
Agilent Seahorse XF Imaging and Normalization FAQs
Agilent Seahorse XF Imaging and Normalization FAQs
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We have used a 20 μM dilution successfully in all validation testing. Which versions of Cytation are compatible? Only the Cytation 1 and 5 are compatible with the XF Imaging and Cell Counting software. How can I export the full resolution images?
Agilent Recorded Webinars
Agilent Recorded Webinars
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Select the Download icon on the right to download a copy of an excel file with a listing of past recorded webinars. *Updated 31 July 2026
This application note demonstrates the ability to image and quantify MMP-14 activity, in addition to tumor invasion, using a 3D tumoroid cell model.
This study shows the high contrast brightfield imaging capabilities of the Cytation 5 to enable convenient and robust long-term kinetic cell proliferation studies.
This application note demonstrates a 3D neurosphere model, composed of hNSCs, to conduct toxicity testing of several potential neurotoxicants.
This application note demonstrates the use of Duolink PLA detection in microplates using the Agilent BioTek Cytation 5 cell imaging multimode reader.
This application brief demonstrates the ability of the Synergy LX multimode reader to measure fluorescence and determine GFP transfection efficiency.
In this application note, chloroquine was used to demonstrate that LUCS assay can be implemented in a “plug-and-play” mode using the Agilent BioTek Cytation 5.
In this application note, the Agilent BioTek Cytation 5 cell imaging multimode reader was used for quantitative assessment of antioxidant activities in living cells.
This application note describes the ability to automate the steps necessary to create, track, and monitor hMSCs during differentiation into chondrocyte lineages.
This application note describes phenotypic and quantitative assessments of 2D and 3D target cell necrotic induction were made using automated live cell imaging.
This application note demonstrates the validation of a combined solution to perform automated chondrocyte differentiation from 3D hMSC spheroids.
This application note evaluates the ability to detect changes in ERK/CREB phosphorylation levels post treatment with Aβ using the SH-SY5Y neuroblastoma cell line.
This application note describes a novel 3D NK cell mediated cytotoxicity (CMC) assay.
This study demonstrates the utility of a 3D spheroid cell culture model, used to elucidate the apoptotic potential of two compounds in two different cell lines.
The alkaline comet assay is a robust method to perform genotoxicity measurements and, with the addition of the 96-well CometSlide and CometChip, analysis is efficiently performed on large sample numbers in a high-throughput format.
Automated brightfield imaging assay enables label-free quantification of T cell and CAR T cell activation through dynamic cluster formation analysis.