Refine Results
- Products
- Applications and Industries
- Content Type
- Analysis Technique
- Analyzed With
- Chemical Group
- Compounds
- Detection Technique
- Injection Technique
- Instrument
- Agency Method
- Inner Diameter
- Length
- Manufacturer Compatibility
- Unit Quantity
- Analysis Platform
- Language
- Publication Date
1 - 20 of 66 results
J&W DB-35ms GC Column, 30 m, 0.25 mm, 0.25 µm, 7 inch cage. This is the most common GC column format compatible with Agilent 5890, 6890, 7820, 7890, 8860, and 8890 series GC systems and all non-Agilent GC systems with a similarly sized oven.
-
Unit:
1 Each
-
Length:
30 m
J&W DB-35MS GC column, 30 m, 0.25 mm, 0.25 µm, with smart key, for 8890 GC system
-
Unit:
1 Each
-
Length:
30 m
J&W DB-35ms GC Column, 30 m, 0.25 mm, 0.25 µm, LTM column module. This format is only compatible with Agilent Low Thermal Mass Series Rapid Heating/Cooling (LTM) Systems.
-
Unit:
1 Each
-
Length:
30 m
J&W DB-35ms Ultra Inert GC Column, 30 m, 0.25 mm, 0.25 µm, 7 inch cage. This is the most common GC column format compatible with Agilent 5890, 6890, 7820, 7890, 8860, and 8890 series GC systems and all non-Agilent GC systems with a similarly...
-
Unit:
1 Each
-
Length:
30 m
Internal Standard Not Showing Up
Internal Standard Not Showing Up
-
community.agilent.com/.../2026_2D00_02_2D00_11_2D00_1308_5F00_etuneTorkelsonNL.pdf community.agilent.com/.../Salmon-FAME-Meth-2-02052026.pdf community.agilent.com/.../70C-Jenkins-FAME-IS-Only.pdf community.agilent.com/.../acqmeth-70C-Jenkins-FAME-IS-Only.txt I previously used a method with DCM and heat, but I was unable to see most FAME which I believe was due to degradation (we are at 7000 ft so I think elevation played a role). However, I was able to see our internal standard (23:0). With the heat issue and trying to move away from DCM we tried a few new methods. Which each new method (listed below) we were able to see the FAME in samples (salmon SRM and olive oil) as expected so that was an improvement, but we were no longer able to see our internal standard. Based on our ability to see similar sized FAME I do not believe it is an instrument issue, but I am not able to find anything about why our internal standard is not showing up. For preparation of the internal standard we dissolved 20.7 mg 23:0 into 100 mL of hexane, but I have a feeling more needs to be done but I have no idea what. I’ve attached the chromatograms for the internal only (70C Jenkins FAME IS Only) and one where the internal did not show up but other FAME did (Salmon Fame Meth 2) along with the latest tune report and the GC-MS method we are using. It is an Agilent 8890 GC/5977B MS with a HP-5MS UI column. While we do have a FATWAX column I regularly run analyses for neutral lipids and the GC is used by other so I would prefer not to regularly change the column. Method 1 (suggestion from Paul Salverda) Add 80 uL of 23:0 standard to tube and evaporate with nitrogen Add 5 mL hexane, vortex Add 200 uL 2M KOH in MeOH, vortex Take top layer, evaporate with nitrogen Method 2 (Agilent application note for milk) Add 80 uL of 23:0 standard to tube and evaporate with nutrogen Add 10 mL hexane, 1 mL sodium methoxide, vortex 1 min Take top layer, evaporate with nitrogen Method 3 (Jenkins, 2010) 100 uL 23:0 standard, evaporate under nitrogen Add 2 mL sodium methoxide (0.5M), vortex Incubate at 50C for 10 min, cool 5 min Add 3 mL 5% methanolic HCl, vortex Incubate at 80C (we tried multiple temperatures) for 10 min, cool 7 min Any suggestions/insights would be greatly appreciated.
Compare
J&W DB-35ms Ultra Inert Intuvo GC column module, 30 m, 0.25 mm, 0.25 µm. (compatible only with the Intuvo 9000 GC System)
-
Unit:
1 Each
-
Length:
30 m
Ideal for confirmation analysis, virtually equivalent to a (35%-phenyl)methylpolysiloxane, with excellent inertness and low bleed.
Various GC columns with a Smart Key included, made specifically to fit the 8890 GC.
J&W 5Q columns combine industry-leading Ultra Inert performance with Ultra Low-Bleed technology, delivering the new standard for GC/MS column performance and productivity.|If your configuration is not available, please request a custom column with Smart Key through the Custom Column Shop.
Improved inertness and exceptionally low bleed, with great peak shapes for challenging active analytes such as pesticides and drugs of abuse.
Provide excellent inertness, low-bleed performance, and detection limits with the Intuvo 9000 GC system.
Offers rapid temperature programming rates, faster column times & shorter analytical cycle times than air-bath GC oven techniques. Available for 7890 and 6890 Series LTM GC systems.
