심혈관 연구

Application Notes

Assessing functional metabolism using cells sourced from blood

PDF/ Found In: Application Notes
Date : 04 Jan 2017
File Size : 643.12 KB

Measuring glycolytic function in cells

PDF/ Found In: Application Notes
Date : 08 Mar 2017
File Size : 1.07 MB

Literature

Application Notes

Analysis of Phosphorylated STAT Protein Signaling in Lymphocytes Using Flow Cytometry

Examines the phosphorylation of signal transducer and activator of transcription (STAT) proteins in response to cytokine stimulation using a phosphoflow technique.

Application Notes
English
27 Nov 2019
1.13 MB
PDF

Measuring glycolytic function in cells

Real-time, kinetic measurement of glycolysis connects energy pathways to cell growth and proliferation

Application Notes
English
08 Mar 2017
1.07 MB
PDF

Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia

Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment

Application Notes
English
03 Jan 2017
744.28 KB
PDF

Measuring Fatty Acid Oxidation with the Seahorse XF Analyzer of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test

Measuring Oxidation of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test

Application Notes
English
22 Dec 2016
1.13 MB
PDF

UHPLC-MS/MS Triple Quadrupole Analysis of Anthocyanin Metabolites in Human Plasma

Anthocyanins are studied in human dosing of strawberry drink by LC/QQQ at low levels in plasma. SPE is to be superior for sample prep and conditions are given.

Application Notes
English
04 Mar 2016
303.27 KB
PDF

Promotions

SNPing in and out of the stem cell genome with CRISPR/Cas9 to investigate cardiovascular disease

In this webinar, Dr. Chris Denning discusses short stories around his research, particularly around the use of Cas9/CRISPR to modify the genome of hiPSC-CMs, along with applications of Agilent Seahorse XF and CardioECR to understand more about disease and variation in the human population. Cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) provide a powerful tool for modelling impact of disease and drugs on structure and function. The presentation will also provide early data on the use of Cas9/CRISPR reporter lines to conduct large scale screens of compounds that may be useful in inducing cardiomyocyte maturation.

eSeminar/Training

Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress

A particularly useful and straight-forward protocol has been to add in series an inhibitor of the ATP synthase (oligomycin), an uncoupler (FCCP) and a respiratory chain inhibitor (antimycin or rotenone) to obtain mitochondrial profile of cells under basal and stressed conditions.

eSeminar/Training

Importance of the Mitochondrial Reserve Capacity in Cardiovascular Cells Exposed to Oxidative Stress.

Reactive oxygen and nitrogen species are part of normal metabolism and play prominent roles in cardiovascular (patho) physiology. Interestingly, these reactive species or their secondary products covalently modify proteins, thereby modulating enzyme function or eliciting redox signaling. Mitochondria, in particular, are prominent sources of such reactive species and are hotspots for oxidative protein modifications.

eSeminar/Training

Cardiovascular Research

Exploring Mitochondrial Function and Cardiovascular Research

Assessing functional metabolism using cells sourced from blood

Measuring glycolytic function in cells

Analysis of Phosphorylated STAT Protein Signaling in Lymphocytes Using Flow Cytometry

Measuring glycolytic function in cells

Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia

Measuring Fatty Acid Oxidation with the Seahorse XF Analyzer of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test

UHPLC-MS/MS Triple Quadrupole Analysis of Anthocyanin Metabolites in Human Plasma

SNPing in and out of the stem cell genome with CRISPR/Cas9 to investigate cardiovascular disease

Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress

A particularly useful and straight-forward protocol has been to add in series an inhibitor of the ATP synthase (oligomycin), an uncoupler (FCCP) and a respiratory chain inhibitor (antimycin or rotenone) to obtain mitochondrial profile of cells under basal and stressed conditions.

Importance of the Mitochondrial Reserve Capacity in Cardiovascular Cells Exposed to Oxidative Stress.

Energy Pathways Video

Measuring Cell Metabolism - Transient Micro-chamber Video