Poroshell 300

Poroshell 300

Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. But Poroshell columns use a unique particle made with a layer of porous silica on a solid core of silica. This reduces the diffusion distance for proteins making practical rapid HPLC separations of peptides and proteins up to 500 – 1,000 kDA. Poroshell 300SB C18, C8, C3, and 300Extend columns provide excellent stability and selectivity choices with TFA and formic acid mobile faces. Poroshell 300Extend C18 columns can be used at varying pH levels for unique separations and additional selectivity choices.

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Biomolecule Separations

특징

UHPLC separations of biomolecules with superficially porous particles
300Å pore provide high efficiency and recovery with proteins (up to 1,000 kDa) and monoclonal antibodies
Achieve long lifetime at low pH with Poroshell 300SB; at high pH with 300Extend-C18
Optimize recovery and selectivity with four different bonded phases – 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18

지원

사용자 설명서

Agilent BioHPLC and AdvanceBio Reversed-Phase Columns User Guide

This user guide provides general information for the Agilent BioHPLC and AdvanceBio reversed-phase columns.

사용자 설명서
English
28 Oct 2020
1007.45 KB
PDF

툴

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Tips & Tools

5989-0683EN

TIPS & TOOLS

Further information can be found in the following publications:

Rapid HPLC Analysis of Monoclonal Antibody IgG1 Heavy Chains Using ZORBAX Poroshell 300SB-C8 (publication # 5989-0070EN)

Use of Temperature to Increase Resolution in the Ultrafast HPLC Separation of Proteins with ZORBAX Poroshell 300SB-C8 HPLC Columns (publication # 5989-0589EN)

Using the High-pH Stability of ZORBAX Poroshell 300Extend-C18 to Increase Signal-to-Noise in LC/MS (publication # 5989-0683EN)

Bonded Phase	Pore Size	Temp Limits*	pH Range	Endcapped	USP Designation
Poroshell 300SB-C18	300Å	90 ºC	1.0-8.0	No	L1
Poroshell 300SB-C8	300Å	90 ºC	1.0-8.0	No	L7
Poroshell 300SB-C3	300Å	90 ºC	1.0-8.0	No	L56
Poroshell 300Extend-C18	300Å	40 ºC above pH 8 60 ºC below pH 8	2.0-11.0	Yes	L1

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Agilent BioHPLC and AdvanceBio Reversed-Phase Columns User Guide

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