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T3 & T7 Polymerases - Details & Specifications

Unit Definition

One unit will catalize the incorporation of 1.0 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C

Purity

The absence of contaminating DNase and RNase activity is confirmed by analyzing the integrity of RNA transcripts on a denaturing polyacrylamide gel

Reaction Conditions

40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 10 units enzyme in a 25 µL volume. Incubate 30 minutes at 37°C

Storage Buffer

20 mM KPO4 (pH 7.7), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, and 50% glycerol (v/v)

For Research Use Only. Not for use in diagnostic procedures