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Peptide Mapping and Post-Translational Modifications

Peptide Mapping and Post-Translational Modifications

Fast, Sensitive and Accurate Methods for Protein Peptide Mapping and Post-Translational Modifications Analysis

Biotherapeutics such as monoclonal antibodies (mAbs) represent one of the fastest growing classes of protein-based drugs in the rapidly growing biopharmaceutical industry. Due to the heterogenous nature of protein drugs, comprehensive analytical characterization is required. Peptide mapping is used to perform an extensive characterization of a protein drug, providing primary amino acid sequence confirmation as well as the identification and quantification of post-translational modifications (PTMs) such as deamidation, oxidation, truncation, phosphorylation, methylation, nitrosylation, ubiquitination, lipidation, glycosylation, and isomerization.

Mapping peptides is critical as even a single amino acid difference between two peptides, as a result of mutation or post-translational modification, alters the physical properties of the peptide and potentially the safety and/or effectiveness of the drug product. Therapeutic proteins are first digested by proteases and then analyzed using LC/MS/MS techniques. Optimized and reliable sample preparation is the key first step toward reproducible peptide maps with minimum artifacts introduced in the process. Ultra-high performance LC and reversed phase chemistries enable high resolution and reproducible separation for the digests. Sensitive and accurate Q-TOF mass spectrometry provides confirmation of peptide sequences and PTM identification, including disulfide maps and location of PTMs.

You need thorough and accurate peptide maps to fully understand your protein. You need the ability to also identify and quantify degradation products and PTMs that can impact protein activity. You need automation and speed at every step to maximize your productivity, yet you have to be completely confident in your analysis. You also need intuitive software to simplify the data analysis process and translate the instrument results to the answers you are looking for.

Agilent offers a comprehensive portfolio of methods and technologies for the highly accurate confirmation of protein sequence, identification of modifications, and routine protein fingerprint monitoring for QA/QC. Agilent provides samples-to-answers solutions, including fast and automated sample preparation, LC separation, robust biocolumns, sensitive mass spectrometry data acquisition, and data analysis software for highly sensitive and accurate peptide mapping and the simultaneous identification and quantification post-translational modifications. You can trust Agilent’s innovative samples-to-answers solutions to deliver accurate and reproducible results and streamline your workflow.

For Research Use Only. Not for use in diagnostic procedures.

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