Agilent’s industry-leading metabolomics portfolio just got better!
Agilent continues to create innovative solutions to address the challenges of metabolic research from sample prep through data analysis. The new Bravo Metabolomics Sample Prep Platform extracts metabolites from plasma samples and incorporates room temperature quenching with lipid removal. The new 6546 LC/Q-TOF delivers sensitivity, resolution, dynamic range, and isotopic fidelity to advance metabolomics research. The new MassHunter Lipid Annotator rapidly and accurately annotates lipid MS/MS spectra to accelerate lipidomics research. The new Mass Profiler Professional lipidomics workflow provides more efficient and consistent answers.
These exciting new products expand Agilent’s comprehensive portfolio of cutting-edge instruments, informatics solutions, consumables and workflows to accelerate your metabolomics research.
6546 LC/Q-TOF – Turbocharge your data
The new Agilent 6546 LC/Q-TOF delivers the industry-best combination of resolution and dynamic range designed to meet the challenges of metabolomics research. TOF technology is an excellent choice for discovery metabolomics because it provides high resolution, high mass accuracy and outstanding isotope ratio fidelity - and maintains this performance even as acquisition rate increases. Isotope ratio fidelity is critical in qualitative flux analysis.
Bravo Metabolomics Sample Prep Platform – Consistent and reproducible results
The newBravo Metabolomics Sample Prep Platform for extracting metabolites from plasma samples provides consistent and reproducible results for translational research. This standardized plasma sample preparation incorporates room temperature quenching and Captiva EMR—Lipid removal technology. Agilent Bravo Metabolomics Workbench software uses a form-based interface and requires no complex programming, allowing any user to walk up and start the automated sample preparation.
MassHunter Lipid Annotator software – Fast lipid annotation
The newMassHunter Lipid Annotator software generates fast in silico spectral matches to accurately annotate lipid MS/MS spectra. Lipid Annotator provides a high-level review of annotation results including a pie chart view of lipid classes and a configurable scatter plot of the features. The lipid match detail tab allows an in-depth review of the annotation results from a lipid sum composition table. Selecting an entry in that table interactively displays possible lipid constituents with associated spectral match results including a mirror plot of the MS/MS spectral match.
Lipidomics Workflow for MPP – Enabling lipidomics
The new Agilent lipidomics workflow in Mass Profiler Professional (MPP) offers simple lipid-class normalization, a useful lipid matrix plot, and an informative Kendrick mass defect plot. This lipidomics workflow is designed to meet some unique challenges in lipid results from either Agilent Q-TOF or IM Q-TOF data. Designed for processing and interpreting complex data, MPP software includes principal component analysis, ANOVA, clustering algorithms, correlation analysis, and class prediction, to efficiently turn large sample sets into meaningful information. MPP analysis can be automated using an intuitive visual tool for creating simple or complex processing methods for routine analysis.
Agilent offers two targeted metabolomics solutions for use with the Agilent 6470 LC/TQ system. The Agilent Metabolomics dMRM Database and Method enables straightforward implementation of an optimized LC/MS/MS analysis of >215 central carbon metabolites. Benefit from a curated database with retention times, optimized MS/MS acquisition parameters, and a data acquisition and analysis method.
The AbsoluteIDQ p180 Kit from biocrates quantifies metabolites that reflect diverse physiological processes such as cell cycle control, mitochondrial function, and inflammation. The ready-to-use kit provides a simple automated workflow for the analysis of amino acids, biogenic amines, acylcarnitines and lipids.
The biocrates MxP Quant 500 kit quantifies metabolites with coverage of up to 630 metabolites from 26 biochemical classes, bringing advanced and reproducible metabolomics technology to academic and clinical researchers. The Agilent 6495C triple quadrupole LC/MS system combined with the MxP Quant 500 kit provides high throughput and excellent reproducibility. Only small sample volumes (10 μL) are required for straightforward implementation of a targeted metabolomic method.
Agilent provides innovative discovery and targeted metabolomics solutions based on our powerful portfolio of instruments, databases, and informatics tools. Agilent has developed robust workflows for performing global metabolite profiling using TOF-based mass spectrometry with GC, LC, CE, and SFC separation. For targeted metabolomics, Agilent’s triple quadrupole LC/MS and GC/MS systems provide the sensitivity, reproducibility and robustness needed to handle large sample sets. Agilent’s leading metabolomics software portfolio includes data mining, statistical analysis, compound identification, pathway visualization and qualitative flux analysis.
An automated metabolite extraction
Presented by Jennifer Van Eyk, Director, Advanced Clinical Biosystems Research Institute and Weston Spivia, Research Associate III, Advanced Clinical Biosystems Research Institute, Cedars Sinai