Small RNA Analysis - Details & Specifications
The Small RNA kit for the Agilent 2100 Bioanalyzer provides a fast and sensitive analysis to resolve small nucleic acids, typical applications are:
- Analyze miRNA directly in total RNA or in enriched samples
- Compare small RNA regions in extracts and tissues
- Separate miRNA from other small RNAs.
- Evaluate miRNA yields while optimizing extraction parameters
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The electropherogram shows the analysis of the small RNA content (75 ng/µL) in a thymus total RNA sample. The miRNA and the small RNA region are indicated. (Application Note 5989-7870EN) |
| Analytical specifications | Small RNA kit |
| total RNA | |
| Quantitative range | 50-2000 pg/µL of purified miRNA in water |
| Qualitative range | 50-2000 pg/µL of purified miRNA in water |
| Size range | 6-150 nt |
| Sensitivity (S/N>3)1 | 50 pg/µL in water |
| Quantitation reproducibility (within a chip) |
25% CV |
| Quantitation accuracy | - |
| Maximum sample buffer strength | 10 mM Tris 0.1 mM EDTA |
| Physical specifications | |
| Analysis time | 30 minutes |
| Samples per chip | 11 |
| Sample volume | 1 µL |
| Kit stability | ≥4 months at 4°C |
| Kit size | 25 chips 11 samples/chip = 275 samples/kit |
1Measured for the 40 nt fragment of the Small RNA ladder
For Research Use Only. Not for use in diagnostic procedures