SideStep Lysis and Stabilization Buffer allows you to go from mammalian cells to qPCR or QRT-PCR experiments without any nucleic acid purification steps./p>
QRT-PCR is an important technique for studying mRNA levels in biological samples. Conventional QRT-PCR experiments include RNA isolation steps in order to protect the mRNA of interest from degradation by cellular RNases and to remove inhibitors of reverse transcriptase from the sample. The process of RNA isolation is tedious and time-consuming, particularly when processing large numbers of samples. In addition, RNA is subject to loss during isolation procedures, which is especially problematic when working with small samples and low-abundance RNAs.
Cell lysate storage at least 6 months at -20°C without RNA degradation
All of our SideStep QRT-PCR kits include are patent pending lysis and stabilization technology which uniquely delivers RNA stabilization in cell lysates. Other products in this class simply offer sample lysis. Because RNA is stabilized immediately in the SideStep buffer, samples can be analyzed now or kept in the freezer until all samples have been collected. This is an ideal format for screening large amounts of samples, which can be stored until data is collected, and then further processed. We created cell lysates using the SideStep buffer and stored them 6 months and observed no appreciable loss or degradation of RNA as determined by Agilent bioanalyzer. Additionally, we functionally tested these lysates in QRT-PCR and observed no shift in Ct value vs. fresh lysates, indicating that RNA is stable and intact while stored in the SideStep buffer. (Figure 1)
For Research Use Only. Not for use in diagnostic procedures