Agilent's RNase Block ribonuclase inhibitor is a recombinant form derived by molecular cloning technology.
Any application where eukaryotic RNase contamination is a potential problem
In vitro RNA translation
RNase Block does NOT inhibit:
T7, T3, or SP6 RNA polymerases
Taq DNA polymerase
AMV or MMLV reverse transcriptases
Unit Definition: One unit will inhibit 50% of the activity of 5 ng of RNase A.
Purity: Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts with 40 U of RNase Block Ribonuclease Inhibitor for 1 hour at 37°C and by checking for the absence of degradation.
Storage Buffer: 20 mM HEPES-KOH (pH 7.6), 50 mM KCI, 8 mM DTT and 50% (v/v) glycerol
For Research Use Only. Not for use in diagnostic procedures