RNace-it Ribonuclease Cocktail is a mixture of two RNases: RNase A and a nonspecific RNAse from Aspergillus oryzae. The combined action of these ribonucleases results in the degradation of RNA into small oligoribonucleotides (Figure 1).
Figure 1: RNA Degraded with RNace-It Ribonuclease Cocktail
Lane 1: untreated boiling miniprep DNA. Lane 2: boiling miniprep DNA incubated with 20 U of RNace-It cocktail for 5 minutes at 37°C.
Unit Definition: 20 units will degrade the RNA in a 1.5-ml plasmid preparation