Skip to main content

Agilent Seahorse XF Technology News and Information

Measure What's Important to Your Cell

With over 20,000 genes, 200,000 proteins and thousands of pathways, you can't measure everything in a cell at once, but you can measure what provides the energy that drives them—metabolism.
Agilent Seahorse XF Analysis uses label-free technology to detect discrete changes in cell bioenergetics in real-time, providing a window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis. Move beyond analyzing what your cells are, and reveal a clearer picture of what they do.

Real-Time, Label Free,
Live Cell Analysis

Agilent Seahorse XF Technology simplifies cell energy metabolic analysis. Instruments, sensor cartridge, assay kits and software combine seamlessly to provide powerful metabolic data from live cells in real-time.
  • Mitochondrial Functionality
  • Glycolytic Functionality
  • Metabolic Phenotype
  • Cell Fuel Flexibility/Preference
  • Request a Demo

The gold standard in mitochondrial functionality, providing basal respiration, ATP-linked respiration, maximal respiration and spare respiratory capacity.

Get a complete profile of cellular glycolysis. Quantify glycolytic rate and pathway capacity.

Determine how energetic or quiescent your cells are, their preferred energy pathway and how they utilize these pathways in response to stimulation.

Understand cell fuel/substrate metabolism from three fuel sources. Identify preference, flexibility and fuel liability.

Informative XF Technology Videos

These videos provide an overview of how Agilent Seahorse XF Technology delivers the most comprehensive assessment of cell metabolism.

Seahorse Educational Resources

Seahorse Webinars How Seahorse Analyzers Work
Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well.
All Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes.
Seahorse Webinars Seahorse XF Technology Webinars
Seahorse XF technology webinars are available on a wide range of topics.
Seahorse Cell Reference Seahorse Cell Reference Database
Search the list of Agilent Seahorse XF publications by research area, cell type, cell line, xf assay and author.
Then download your results.
Seahorse Publications Seahorse Publications Database
Browse the full list of publications using Agilent Seahorse XF data.

Powerful Cell Energy Metabolic Analysis

Find the right instrument for your workflow and throughput, the right assay for your biological question and interpret results with our powerful software.
Agilent offers three configurations of XF Instruments, depending on your throughput needs and sample type.
Assays & Consumables
Easy to use assay kits, FluxPaks, media and reagents simplify Seahorse XF Analysis. Search by instrument type.
XF Software
Design, run and analyze XF assays with our complete suite of software products.

Solutions for your applications

Mitochondrial function and glycolysis play critical roles in a variety of cellular processes, including cellular activation, proliferation, differentiation, cell death, and disease progression. Discover how real-time, live cell analysis can provide a complete picture of what's driving your cell's phenotype and function.

Cancer Research

Cancer cells reprogram their metabolism in order to generate the energy and building blocks they need to proliferate. Agilent Seahorse XF technology provides a window into the Warburg effect, fuel usage and other events that drive tumor cell biology.

Immunology Research

Immunometabolism has emerged as a crucial component to understand the connection between metabolic pathways and immune responses. Seahorse XF Technology enables researchers to understand and perturb immune activation and response in real-time through metabolic measurements.

Obesity, Diabetes and Metabolic Disorders

Research into metabolic profiles and changes is enabling insight into browning, substrate and nutrient utilization, and inflammation. Agilent Seahorse XF technology provides the capability to examine functional metabolism in models of cellular metabolic syndromes.

Neurobiology Research

Researchers are using Agilent Seahorse XF technology to explore metabolic changes in neuronal systems, using live cells, in real-time, requiring less biological samples than traditional assays.

Stem Cell Research

Metabolic shift in induced pluripotent stem cells (iPSCs), has a significant importance in establishing pluripotent identity. Using Agilent Seahorse XF technology, researchers can predict the ability of somatic cell reprogramming to iPSCs, and how PSCs alter their metabolism during the differentiation process to terminally differentiated cells.


Agilent Seahorse Community Center

Worldwide Technical Support
USA and Canada
1-800-227-9770; select option 3 then 8
UK: 0800 096 7632
Denmark: +45 8830 5083
Germany: 0800 180 66 78
Netherlands: 0800 022 7243
Other EU: +45 3136 9878
For Research Use Only. Not for use in diagnostic procedures.