QuikChange Lightning: the New Gold Standard for Site-Directed Mutagenesis
Since 1996, the QuikChange method has provided researchers with an easy 3-step process to create point mutations, deletions, and insertions in a variety of plasmid types. The latest additions to this product line, the QuikChange Lightning Site-Directed Mutagenesis Kit and QuikChange Lighting Multi Site-Directed Mutagenesis Kit, have an accelerated protocol (one hour for a single site; three hours for multiple sites) that maintains the same mutation efficiency, reliability, and accuracy established by the original QuikChange and QuikChange II kits.
Efficient mutagenesis >85% for 1 site, >55% for 3 sites
Non-PCR method Eliminates unwanted second site-errors
Fast high-fidelity polymerase Short extension times—30 sec/Kb