Access detailed information at the intact protein level, automatically confirm protein sequence at the peptide level, and understand post-translational modifications—all with this complete workflow solution.
From Sample Preparation to Data Analysis: Agilent Biopharma Workflow
Characterizing proteins requires multiple approaches to accessing varying levels of information. Learn how sample preparation, LC/MS, and software innovations can help you meet your protein characterization challenges.
SWARM Autotune for LC/Q-TOF
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Additional resources for preparing, separating, detecting, and analyzing biomolecules
6545XT AdvanceBio LC/Q-TOF brochure
Whether you are analyzing a single sample or a freezer full of plates, the 6545XT provides methods for each level of analysis to get you up and running fast.
Be confident at the intact protein level with reliable quantitation of major and minor glycoforms and spot-on mass assignments for mAb and subunit analyses. You also get the sensitivity you need to easily detect large noncovalent complexes or antibody-drug conjugates under native conditions.
Whether you are performing routine sequence confirmation or faced with the challenge of hard-to-detect peptides, the 6545XT along with Agilent MassHunter software is ready to help. With the MassHunter BioConfirm module, you can quickly confirm sequence coverage—automatically—and with MassHunter Walkup, you can give access to anyone in the lab. Useful acquisition tools such as iterative MS/MS and real-time sequence matching can help you pull out low-level peptides and improve coverage for hard-to-detect peptides.
To fully understand the precise composition of your biotherapeutic, you need to see more than just the backbone structure. You need to know what modifications and variations are occurring during expression. Whether that means characterizing levels of oxidation, localizing deamidation sites, or profiling glycan structures, the 6545XT is designed to help. Autotune unlocks the full potential of the system by flexibly optimizing for large proteins or small labile structures such as glycans.