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Agilent offers the complete biopharma workflow - from sample prep to data analysis

Designed for Biopharma

Agilent 6545XT AdvanceBio LC/Q-TOF system

Increase your biopharma workflow efficiency

Agilent provides complete workflows for multiple biopharma applications—from sample prep to data analysis. The 6545XT AdvanceBio LC/Q-TOF is designed to be an integral part of these workflows, delivering quick, quality results you can count on, time after time.

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Streamline Your BioPharma Workflow

Access detailed information at the intact protein level, automatically confirm protein sequence at the peptide level, and understand post-translational modifications—all with this complete workflow solution.

SWARM Autotune
for LC/Q-TOF

Get expert results every time, specific to your application.

Additional resources for preparing, separating, detecting, and analyzing biomolecules

6545XT AdvanceBio LC/Q-TOF brochure

Whether you are analyzing a single sample or a freezer full of plates, the 6545XT provides methods for each level of analysis to get you up and running fast.

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AdvanceBio Peptide Plus BioLC columns brochure

These columns deliver the speed, sensitivity, and efficiency you need to quickly confirm and identify target proteins and peptides.

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AssayMAP protein sample preparation brochure

An easy-to-use yet flexible automation solution for preparing protein and peptide samples for LC/MS analysis.

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MassHunter BioConfirm software brochure

This software makes characterization of protein therapeutics fast and routine.

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Application notes for multiple workflows in biopharmaceutical characterization

Intact protein analysis

Be confident at the intact protein level with reliable quantitation of major and minor glycoforms and spot-on mass assignments for mAb and subunit analyses. You also get the sensitivity you need to easily detect large noncovalent complexes or antibody-drug conjugates under native conditions.

Application notes


Peptide mapping

Whether you are performing routine sequence confirmation or faced with the challenge of hard-to-detect peptides, the 6545XT along with Agilent MassHunter software is ready to help. With the MassHunter BioConfirm module, you can quickly confirm sequence coverage—automatically—and with MassHunter Walkup, you can give access to anyone in the lab. Useful acquisition tools such as iterative MS/MS and real-time sequence matching can help you pull out low-level peptides and improve coverage for hard-to-detect peptides.

Application notes

Technical overview

Post-translational modification

To fully understand the precise composition of your biotherapeutic, you need to see more than just the backbone structure. You need to know what modifications and variations are occurring during expression. Whether that means characterizing levels of oxidation, localizing deamidation sites, or profiling glycan structures, the 6545XT is designed to help. Autotune unlocks the full potential of the system by flexibly optimizing for large proteins or small labile structures such as glycans.

Application notes

On-demand webinar

Three Steps to Glycan Success. Learn how a single set of robust and easy-to-learn tools can tackle multiple levels of protein characterization. Register here

Productivity kit

Confidently confirm protein molecular weight and determine modifications. Kit includes two in-depth product brochures, application notes, and a poster. Download here

Be Agilent sure in biopharma

There has never been a better time to streamline your biopharma workflows, with technology from Agilent. We offer innovative solutions every step of the way. Learn more

For Research Use Only. Not for use in diagnostic procedures.