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Aggregate and Fragment Analysis Solutions

Aggregate and Fragment Analysis Solutions

Agilent Biocolumns critical quality attributes (CQAs)


illustration Aggregate and fragment analysis

Size exclusion chromatography

Accurate, precise quantitation for a broad range of biomolecule separations

AdvanceBio SEC 1.9 µm columns

High productivity for fast aggregate and fragment separations

Attribute Advantage
Optimized 1.9 µm silica particles No secondary interactions
Extended column lifetime Minimal downtime, maximum cost savings
Reproducible column performance Confident CQA analysis
Faster separations Greater productivity

AdvanceBio SEC 2.7 µm columns

Versatile performance for routine and challenging applications

Attribute Advantage
Hydrophilic polymer coating Avoid secondary interactions
Increased analytical speed Meet vital deadlines
Higher reproducibility Reduce rework
Greater sensitivity Quantitate aggregates, even at low levels

Bio SEC-3 and Bio SEC-5 columns

Extra wide pore and scale-up options

Attribute Advantage
Compatibility with most aqueous buffers Method flexibility
Wide range of pore size options, including 1000 Å and 2000 Å Options for everything from peptides to VLPs
Analytical and semiprep dimensions Easy scale-up or scale-down

Try Agilent Biocolumns for 60 days risk-free

If any Agilent HPLC column doesn't meet your expectations, simply return the column within 60 days of purchase and receive a full credit. Use promotion code 8642.*

*Offer not valid in all countries. Check the promotions page for all offers available in your area.

New Agilent AdvanceBio SEC 1.9 µm columns

Agilent AdvanceBio SEC 1.9 μm columns

Fast separations for:

  • mAb aggregates and fragments
  • Antibody drug conjugate (ADC) aggregation
  • Low molecular weight fragments
Learn more

Application compendium for aggregate and fragment analysis

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AdvanceBio SEC video

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AdvanceBio columns

Agilent AdvanceBio columns deliver results you can be sure of when analyzing complex biotherapeutic molecules. Use them to monitor purity, potency, and other CQAs. Discover the full range for:

  • Titer determination
  • Glycan analysis
  • Charge variant analysis
  • Amino acid and cell culture analysis
  • Peptide mapping
  • Aggregate/fragment analysis
  • Intact and subunit analysis

Webinar series

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Coming soon—15 May 2019

Webinar on Fast Aggregate and Fragment Analysis Using High-Efficiency Size Exclusion Chromatography

Access webinars
For Research Use Only. Not for use in diagnostic procedures.