Aggregate and Fragment Analysis Solutions

Accurate, precise quantitation for a broad range of biomolecule separations

Agilent AdvanceBio SEC 1.9 µm columns - high productivity for fast aggregate and fragment separations

Modified surface chemistry minimizes secondary interactions
Optimized pore size and volume ensures high resolution and efficiency
Extended column lifetime for minimal downtime and maximum cost savings
Reproducible column performance for confident CQA analysis
Faster separations for greater productivity

Agilent AdvanceBio SEC 2.7 µm columns - versatile performance for routine and challenging applications

Hydrophilic polymer coating minimizes secondary interactions
Increased analytical speed to meet vital deadlines
Higher reproducibility reduces rework
Greater sensitivity to quantitate aggregates, even at low levels

Agilent Bio SEC-3 and Bio SEC-5 columns - extra wide pore and scale-up options

Compatibility with most aqueous buffers for method flexibility
Wide range of pore size options including 1000 Å and 2000 Å, suitable for everything from peptides to VLPs
Analytical and semiprep dimensions allow easy scale-up or scale-down

Aggregate and Fragment Resources

Application Compendium

'How To' guide, applications, and more.

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Workflow Ordering Guide

Analyzing Monoclonal Antibody (mAbs) and mAb-Derived Therapeutics Using Native SEC-MS

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Workflow Ordering Guide

Analyzing Monoclonal Antibody (mAb) Fragments and Dimers Using Size Exclusion Chromatography (SEC)

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Webinar

Recorded webinar Native and Denaturing Mode SEC Analysis of Biotherapeutics.

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Video

See how Agilent's AdvanceBio SEC columns eliminate slow separations, poor resolution, and other roadblocks to successful mAb analysis.

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AdvanceBio SEC 120 Å 1.9 µm columns

High-resolution analysis of peptides and proteins with AdvanceBio SEC 120 Å 1.9 µm columns

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AdvanceBio SEC 200 Å 1.9 µm columns

Fast separations for aggregates and fragments with AdvanceBio SEC 200 Å 1.9 µm columns.

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AdvanceBio SEC 2.7 µm columns

End uncertainty in monoclonal antibody characterization.

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Try AdvanceBio columns for 60 days risk-free

If any Agilent HPLC column doesn't meet your expectations, simply return the column within 60 days of purchase and receive a full credit.*

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AdvanceBio columns

Agilent AdvanceBio columns deliver results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes (CQA). Discover the full range for:

Titer determination
Glycan analysis
Charge variant analysis
Amino acid and cell culture analysis
Peptide mapping
Aggregate/fragment analysis
Intact and subunit analysis

Interested in webinars? Watch our AdvanceBio webinars on-demand: www.agilent.com/chem/advancebio-webinars

Unsure which AdvanceBio column is best suited for your application? Try our new eSelector tool: www.agilent.com/chem/eselectortools

Confidently Characterize, Identify, and Quantify Protein Aggregates

The safety and efficacy of protein-based therapeutic agents are highly dependent on the correct primary, secondary, ternary, and quaternary structure of the molecule. Aggregation can occur at any point during manufacturing. Agilent has a complete portfolio of solutions for protein aggregation analysis including the new InfinityLab Bio LC portfolio, LC/MS, and software suites.

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* Use promotion code 1754 (Europe and India), 1677 (US and Canada), or 1565 (Asia Pacific). Offer not valid in all countries. Terms and conditions apply. Promotional products cannot be substituted with other Agilent products. Customer's standard purchase agreement terms and conditions apply to this offering. Custom columns are not eligible.

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