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AdvanceBio HIC

Robust Hydrophobic Interaction Chromatography of Intact Native Proteins

NEW Agilent AdvanceBio HIC columns

Identify and quantify critical quality attributes (CQAs) of biologically active proteins at the intact level

Now you can use hydrophobic interaction chromatography (HIC) instead of reversed-phase chromatography to resolve CQAs from parent molecules with a small difference in hydrophobicity. And you don’t have to compromise performance or productivity.

Agilent AdvanceBio HIC columns—the latest addition to our AdvanceBio family—​deliver high-performance CQA separations of native proteins at the intact level. They are designed and engineered to:

Be confident that all your impurities are resolving. AdvanceBio HIC columns are specifically designed for separating heterogeneous biotherapeutic proteins.
  • Maintain sample integrity by performing your analysis on an intact native molecule
  • Get improved accuracy and quantitation of CQAs with an innovative particle technology that provides better peak shape with lower back pressure
  • Get your methods up and running faster with a single chemistry for a wide range of molecules to eliminate multiple column screening
  • Improve productivity, reduce analysis time by 3-fold for time-critical decision making
  • Provide more confidence in your results with a column that provides robust and reproducible separations across multiple sites

NEW Intact Biologics Productivity Kit

Kit includes:

Product brochure

Robust Hydrophobic Interaction Chromatography of Intact Native Proteins

New application notes

  • An AdvanceBio HIC Column for Drug‑to-Antibody Ratio (DAR) Analysis of Antibody Drug Conjugates (ADCs)
  • AdvanceBio HIC: A Hydrophobic HPLC Column for Monoclonal Antibody (mAb) Variant Analysis

On-demand webinar

Hear how novel HIC stationary phases can solve current challenges in determining critical quality attributes of mAbs and ADCs.

Request yours

Identifying and quantifying oxidation in monoclonal antibodies (mAbs)

The Agilent AdvanceBio HIC column efficiently separated the nonoxidized and oxidized form of the mAb molecule.

Time-critical analysis of antibody drug conjugates (ADCs)

With a shorter column, you can reduce the analysis time by 3-fold—and still have sufficient resolution to identify and quantify the ADC DAR value.

For Research Use Only. Not for use in diagnostic procedures.

To download your Intact Biologics Productivity Kit, complete this form.