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Basic Procedures to Run an XFp Assay

Planning Your XFp Assay

Materials Required to Run an XFp Assay
A list of the materials required or recommended to run a successful XFp assay.

Procedures Required the Day Before the XFp Assay

Seeding Adherent Cells in XFp Cell Culture Miniplates
This basic procedure describes recommendations for seeding adherent cell types for an XFp assay in the XFp Analyzer.

Hydrating the Sensor Cartridge for the XFp Analyzer
This basic procedure describes the recommended procedure and conditions for hydrating an XFp Sensor Cartridge before running the XF assay.

Procedures Required the Day of the XFp Assay

Preparing Assay Media for Use in XFp Assays
This basic procedure details the preparation of assay media for use with the XFp Cell Mito Stress Test Kit, and XFp Glycolysis Stress Test Kit.

Washing Adherent Cells in XFp Cell Culture Miniplates
This procedure describes the procedure for replacing growth medium with assay medium for adherent cells grown in XFp Cell Culture Miniplates prior to being assayed using an XFp Analyzer.

Seeding Suspension Cells in XFp Cell Culture Miniplates
This basic procedure describes recommendations for seeding suspension cell types for an XFp assay in the XFp Analyzer.

Loading the XFp Sensor Cartridge with Compounds
This basic procedure provides cartridge loading instructions for XFp assays on the XFp Analyzer.

Running the XFp Assay

New Cell Line Characterization
This basic procedure details the experimental steps to characterize the metabolic phenotype for a cell type of interest, including assessment of optimal density, titration of FCCP and running the XFp Glycolysis Stress Test Kit and XFp Cell Mito Stress Test Kit.



For Research Use Only. Not for use in diagnostic procedures.