Bio-Monolith Protein A and Protein G
Choose the Agilent Bio-Monolith Protein G column for purification of IgG molecules that do not bind to the Agilent Bio-Monolith Protein A.
Product Details
- Analytical separation of all IgG subclasses (human and mouse), Protein G offers alternate selectivity for those IgG molecules that do not bind to Protein A
- Flow-rate independent separations; no diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid
- Extremely fast separations speed up method development time and decrease costs
- Locking in method parameters takes significantly less time and buffer
- Key applications are quantitative determination of IgG (fermentation titer calculation)
| Column Specifications | |
| Description |
The Protein A affinity column is designed for the analytical separation of IgG1 and IgG2 (human and mouse) The Protein G affinity column provides analytical separation of IgG molecules that do not bind Protein A. |
| Key applications | Quantitative determination of IgG (fermentation titer calculation) |
| Dimensions | 5.2 mm x 4.95 mm |
| Column volume | 100 µL |
| Maximum pressure | 150 bar (15 MPa, 2,000 psi) |
| Temperature min/max | Operating : 2-40 °C |
| Storage: 2-8 °C | |
| Recommended pH | Operating range: 2-13 |
| Cleaning-in-place: 1-14 | |
| Materials of construction | Hardware: stainless steel |
| Packing: poly(glycidyl methacrylate-co-ethylene dimethacrylate) highly porous monolith | |
| Color ring identifier | Bio-Monolith Protein A: white, Bio-Monolith Protein G: orange |
| Shelf life / expiration date | 12 months |
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