
Bio-Monolith Protein A and Protein G
Choose the Agilent Bio-Monolith Protein G column for purification of IgG molecules that do not bind to the Agilent Bio-Monolith Protein A.
- Biomolecule Separations
Product Details
- Analytical separation of all IgG subclasses (human and mouse), Protein G offers alternate selectivity for those IgG molecules that do not bind to Protein A
- Flow-rate independent separations; no diffusion, no pores and no void volume make transport between mobile and stationary phase very rapid
- Extremely fast separations speed up method development time and decrease costs
- Locking in method parameters takes significantly less time and buffer
- Key applications are quantitative determination of IgG (fermentation titer calculation)
Column Specifications | |
Description |
The Protein A affinity column is designed for the analytical separation of IgG1 and IgG2 (human and mouse) The Protein G affinity column provides analytical separation of IgG molecules that do not bind Protein A. |
Key applications | Quantitative determination of IgG (fermentation titer calculation) |
Dimensions | 5.2 mm x 4.95 mm |
Column volume | 100 µL |
Maximum pressure | 150 bar (15 MPa, 2,000 psi) |
Temperature min/max | Operating : 2-40 °C |
Storage: 2-8 °C | |
Recommended pH | Operating range: 2-13 |
Cleaning-in-place: 1-14 | |
Materials of construction | Hardware: stainless steel |
Packing: poly(glycidyl methacrylate-co-ethylene dimethacrylate) highly porous monolith | |
Color ring identifier | Bio-Monolith Protein A: white, Bio-Monolith Protein G: orange |
Shelf life / expiration date | 12 months |
- Key Literature
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Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures
In this application note, pre-packed Bio-monolith Protein A columns, part of the AdvanceBio family, were used to demonstrate the quick capture of monoclonal antibody titer
- Application Notes
- English
- 02 Nov 2017
- 440.76 KB
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