Reversed-phase desalting for on-line removal of salt ions before MS detection
Affinity, ion exchange, and size exclusion chromatography are commonly used techniques in the analysis of proteins such as monoclonal antibodies. However, these techniques require aqueous mobile phases that contain non-volatile salts. These non-volatile salts present a problem when using MS detection as they cause signal suppression and can contaminate the MS detector through salt deposition leading to increased maintenance and instrument downtime. AdvanceBio Desalting-RP cartridges solve this problem, enabling fast and efficient on-line removal of salt ions before MS detection. These cartridge style columns can be used on their own on any LC system to desalt collected fractions. Or on an Agilent 1290 Infinity II 2D-LC system as the second dimension to desalt after the first dimension separation.