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A new measure for authenticating cellular models

The Agilent Advantage - Predict Stem Cell Reprogramming and Differentiation Potential In Real-Time
Agilent Seahorse XF technology measures discrete changes in cellular bioenergetics in real-time, enabling researchers to measure gain, maintenance, and loss of pluripotency. Along the differentiation axis, metabolic phenotyping can be used to validate cell models. Achieve a new level of cell characterization, control and efficiency through label-free, real-time analysis. Expand these sections below to learn more about accelerating your stem cell research with XF technology.
Stem Cell Metabolism Link
stem cell marker
Fig 1
Stem cells, somatic cells, and differentiated cells exhibit specific metabolic signatures. Measurable metabolic switching events and resultant preference of glycolytic and/or oxidative respiration energy pathways, occurs at multiple stages when cells become reprogrammed, enter and exit a pluripotent state, begin to differentiate, and terminally differentiate.
Stem Cell Resources
Fig 2  Mouse over for full image.
Reprogramming/Differentiation Efficiency
Early detection of reprogramming and differentiation capacity.
Metabolic energy utilization and preference, characterized before and after cell fate changes occur, identify the metabolic phenotype allowing researchers to predict and confirm cell function, revealing actionable reprogramming and differentiation potential.
pluripotency stability
Fig 3
Entering and Exiting Pluripotency
Early detection of differentiation.
Distinguishing naïve versus primed stem cells is required for optimizing gene targeting effectiveness. Screening for differentiation potential and isolating the commitment stage provides an actionable window for dictating when the time is right to conduct and prompt the next transition stage.
Fig 4
Validate and Verify Cell Models
Confirm function and proximity to terminal differentiation.
Relevant cellular and disease models require complete characterization and verification of lineage specification, cellular function, and performance. Cellular specification and maturity can be confirmed by measuring cellular function and comparing with a parental or primary cell type.
Dive Deeper with standardized cell metabolism assays and powerful software
Agilent Seahorse XF kits and reagents are pre-calibrated, pre-tested and are coupled with powerful report generator software, providing functional metabolic data from live cells at every stage of the reprogramming and differentiation process.
ATP Assay
Measure glycolytic and mitochondrial respiration to determine the ATP production rate.
Glycolytic Rate Assay
Provides a complete glycolytic profile.
Mitochondrial Stress Test Kit
Provides a complete mitochondrial respiration profile.
Worldwide Technical Support
USA and Canada
1-800-227-9770; select option 3 then 8
Europe
UK: 0800 096 7632
Germany: 0800 180 66 78
Netherlands: 0800 022 7243
Other EU: +45 3136 9878
Need more information?
Shop the online store to purchase cartridges, plates, kits, media, and reagents to perform your XF assay. Browse the complete list of all Cell Analysis Seahorse consumable products.
Get in touch with an Agilent Cell Analysis representative to schedule a demonstration and see how Agilent Seahorse XF Analyzers can solve your research needs.
Don't wait to accelerate your research, learn more about XF Technology today!
For Research Use Only. Not for use in diagnostic procedures.