The Agilent Advantage - Predict Stem Cell Reprogramming and Differentiation Potential In Real-Time
Agilent Seahorse XF technology measures discrete changes in cellular bioenergetics in real-time, enabling researchers to measure gain, maintenance, and loss of pluripotency. Along the differentiation axis, metabolic phenotyping can be used to validate cell models. Achieve a new level of cell characterization, control and efficiency through label-free, real-time analysis. Expand these sections below to learn more about accelerating your stem cell research with XF technology.
Learn about Metabolic Phenotyping to Identify Cellular Transitions
During Early Neuronal Differentiation
Stem cells, somatic cells, and differentiated cells exhibit specific metabolic signatures. Measurable metabolic switching events and resultant preference of glycolytic and/or oxidative respiration energy pathways, occurs at multiple stages when cells become reprogrammed, enter and exit a pluripotent state, begin to differentiate, and terminally differentiate.
Stem Cell Resources
An early measure of gain of pluripotency
Upregulation of glycolysis is a requirement for efficient reprogramming. These cellular energy changes proceed the expression of pluripotent markers. The bioenergetic transition of mitochondrial respiration (somatic cell) and glycolytic pluripotency induction can be easily and simultaneously measured using XF technology.
Seahorse XF Real-Time ATP Rate Assay Kit:
Dissect cellular energy plasticity by measuring and quantifying the rate of ATP production calculated from the proportional contribution of glycolysis and mitochondrial respiration.
Determine pluripotency homeostasis and differentiation potential
Investigating pathways and energy requirements that support "stemness" maintenance have led to advances of defining the "pluripotent state". The interplay of proliferation, self-renewal, and differentiation can be better understood through characterization of metabolic phenotype due to the critical roles of glycolysis and mitochondrial respiration.
Pluripotency homeostasis and maintenance can be analyzed by measuring the balance of glycolysis and mitochondrial respiration.
Seahorse XF Glycolytic Rate Assay Kit:
Profile basal glycolysis in real-time and measure the cell's ability to increase (compensate) glycolytic rates for determining the degree of proliferation and pluripotency.
Characterize and validate cellular function and performance
Early detection of loss of pluripotency is essential to determining stem cell's readiness to proceed along the desired differentiation fate of choice. Measuring loss of pluripotency depends on measuring both glycolysis (ECAR or PER; proliferation) and mitochondrial respiration (OCR; specification) energetic pathways simultaneously.
Loss of pluripotency can be detected very early by measuring the metabolic switch from glycolysis to mitochondrial respiration.
As cell's progress along the differentiation axis, specific cellular energy phenotypes can be measured to monitor the progress and success of cell fate transitions. The balance between glycolysis and mitochondrial respiration contributes to cell fate determination. By identifying and isolating these commitment stages, researchers can ensure that they have optimized each step of the differentiation process.
Easily distinguish progenitor versus differentiated cell populations to confirm each cell fate transition and monitor differentiation progress and maturity.
Relevant disease models require complete characterization and validation of cellular function and performance.
The purity of a differentiated cell population and maturity along the differentiation axis can be measured by simultaneous glycolytic (ECAR or PER;
stemness presence) and mitochondrial respiration (OCR; functional specification).
Early detection of reprogramming and differentiation capacity.
Metabolic energy utilization and preference, characterized before and after cell fate changes occur, identify the metabolic phenotype allowing researchers to predict and confirm cell function, revealing actionable reprogramming and differentiation potential.
Entering and Exiting Pluripotency
Early detection of differentiation.
Distinguishing naïve versus primed stem cells is required for optimizing gene targeting effectiveness.
Screening for differentiation potential and isolating the commitment stage provides an actionable window for dictating when the time is right to conduct and prompt the next transition stage.
Validate and Verify Cell Models
Confirm function and proximity to terminal differentiation.
Relevant cellular and disease models require complete characterization and verification of lineage specification, cellular function, and performance. Cellular specification and maturity can be confirmed by measuring cellular function and comparing with a parental or primary cell type.
Dive Deeper with standardized cell metabolism assays and powerful software
Agilent Seahorse XF kits and reagents are pre-calibrated, pre-tested and are coupled with powerful report generator software, providing functional metabolic data from live cells at every stage of the reprogramming and differentiation process.
Measure glycolytic and mitochondrial respiration to determine the ATP production rate.
Seahorse XF Stem Cell Research Brochure
to identify steps within the stem cell workflow where Seahorse XF technology can be used to measure loss of pluripotency, differentiation potential, differentiation progression, and functional confirmation..
Stem Cell Research Application Brief
to concisely learn about the Seahorse XF technology advantage and why metabolic measurements are an important next step to take when characterizing stem cells and their differentiated counterparts.