All living cells have an energy phenotype, which is the utilization of both major energy producing pathways - mitochondrial respiration and glycolysis - to meet their energy demands. An Energy Phenotype comprises a baseline phenotype, a stressed phenotype, and a metabolic potential.
What is a cells' Metabolic Potential?
Metabolic Potential reveals the cells' ability, and preferred pathway, to respond to changes in energy demand due to stress. The difference between the baseline phenotype and the stressed phenotype is the cell's Metabolic Potential. This important parameter can change dramatically depending on gene expression and/or drug treatment; it is a sensitive early indicator that a change in phenotype has taken place.
What is the Seahorse XF Cell Energy Phenotype Test?
The Seahorse XF Cell Energy Phenotype Test is the only single assay that can simultaneously report baseline and maximal mitochondrial respiration and glycolytic activity in live cells. The Seahorse XF Cell Energy Phenotype Test is a reagent kit and analysis method that allows you to rapidly determine the Energy Phenotype of your cells, plot it on an Energy Map, and easily compare metabolic phenotypes across cell types and treatment conditions.
Why should I do the Seahorse XF Cell Energy Phenotype Test?
Mapping your cells' Energy Phenotype provides an easy-to-understand view of the role of energy metabolism in your cells, allowing you to create a 'complete picture' of the disease, response to drug treatment, or subtle differences between cell populations. . It is the easiest and best way to understand which energy pathway live cells prefer and how they utilize these pathways in response to stimulation.
How would I use this test in my research?
Metabolism is inextricably linked to - and may even drive - many pathologies as well as normal cellular functions, so metabolic changes can have a huge impact on cellular systems. The Seahorse XF Cell Energy Phenotype Test is used to generate a holistic, functional view of the metabolic phenotype of your cells. The resulting Energy Map can be used as a routine output of the metabolic consistency between cell preparations, a sensitive compound screening metric, or a powerful tool for understanding the energetic differences between multiple groups. This live cell assay will provide a functional test to support or validate your ongoing genetic and proteomic research.
How does the Seahorse XF Cell Energy Phenotype Test work?
The test measures the utilization of each pathway- mitochondrial respiration and glycolysis - first under the starting medium conditions (Baseline Phenotype) and then upon injection of a stressor mix that induces an energy demand (Stressed Phenotype). The Seahorse XF Cell Energy Phenotype Test Report Generator plots these data on the Energy Map and displays the Metabolic Potential of the cells.
Can the Seahorse XF Cell Energy Phenotype Test be used with any XF Analyzer?
Yes, the Seahorse XF Cell Energy Phenotype Test can be run using any Seahorse XF or XFp Analyzer with the Seahorse XF or XFp Cell Energy Phenotype Test Kit and the Seahorse XF Cell Energy Phenotype Test Report Generator.
There are four possible energy phenotypes that a cell can use.
Quiescent: The cell is not very energetic for either metabolic pathway.
Energetic: The cell utilizes both metabolic pathways.
Aerobic: The cell utilizes predominantly mitochondrial respiration.
Glycolytic: The cell utilizes predominentlyglycolysis.