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Pick your Power - Cell Analysis Product Selection Guide

All Agilent cell metabolism assays measure cell metabolism by looking at oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) – it’s the technology that differs. So it’s a matter of understanding which assay best suits your biological questions, workflow, and throughput goals.

Agilent Seahorse XF analyzers use solid-state sensors to simultaneously measure OCR and ECAR in every well, via the creation of a transient microchamber.

MitoXpress and pH-Xtra assays use soluble, chemically inert, cell impermeable, fluorophores to measure OCR and ECAR in real time, using plate-reader based assays.

Use this handy guide of the workflow differentiators to best understand which assay suits your needs.
MitoXpress and pH-Xtra assays Seahorse XF assays
Detection System TR-F enabled microplate readers.

See MitoXpress Xtra, MitoXpress Intra and pH-Xtra assay User Manuals for details
Agilent Seahorse XF, XFe or XF HS Mini Analyzers

See XFe96, XFe24, and XF HS Mini User Manuals for details
Assay Format Any microplates suitable for TR-F measurements
Suitable for 96 and 384 well formats
XF FluxPaks
Available in 8, 24 and 96-well formats
Sensor Soluble, single analyte/pathway Solid state, dual analyte/pathway
Assay Units fluorescence units - relative rates [qualitative] pmol/min - absolute rates [quantitative]
Assay Design Options Pretreatment
Manual or plate reader injection possible for pH-Xtra
Automated, sequential injection of up to four compounds per well during an experiment
Assay Workflow Same day prep Prep day prior to assay
Cell Seeding Seed as normal for 96 or 384 well microplates. Typically requires 3-6 x 104 cells per 96-well Typically requires 1-2 x 104 cells per 96-well
Cell Prep Monolayer or suspension

No adherence required
Monolayer or anchored 3D

Suspension cells and microorganisms require adherence via spinning and/or plate coatings; 3D requires special plates
Cell Types Moderate-to-active respiring cells and microorganisms Most cell types and microorganisms

See Cell Reference database for details
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