Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is an RNA-dependent DNA polymerase. It is a monomeric protein encoded by the central portion of the MMLV polymerase gene. The enzyme expresses both reverse transcriptase (RT) and RNase H activities.
Moloney Murine Leukemia Virus Reverse Transcriptase has been cloned by several laboratories and varies in the unit per microgram (U/µg) of template recommendation. This may be due to the nonstandardized unit determinations or the inherent differences in the cloned constructs. Stratagenes Moloney Murine Leukemia Virus Reverse Transcriptase has been tested thoroughly with RNA templates ranging from 0.24 to 9.0 kb. For targets up to 6kb, 50U has been optimized to produce full-length clones without detectable hairpin structures on 1-5 µg of template. For targets larger than 6kb, 100U is recommended.
For Research Use Only. Not for use in diagnostic procedures.