pFB-ERV Vector is a receptor expression vector, part of the Complete Control Inducible Mammalian Expression System, designed to provide the tightest control of expression available in mammalian cells. The pFB-ERV vector was derived from the high-titer Moloney Murine Leukemia Virus (MoMLV) vector pFBNeo5 for efficient delivery of the ecdysone receptor proteins VgEcR and RXR.
The ecdysone receptor and the neomycin-resistance open reading frame (ORF) are expressed from a tricistronic message with the neomycin resistance ORF expressed at the end of the message. Thus, maintenance of infected cell lines in G418 ensures expression of the transcript encoding the receptor genes. The tricistronic transcript is expressed from the CMV promoter, which is flanked by unique EcoR I and Fse I sites so that a cell type-specific promoter of interest may be substituted.
The viral promoter within the 3 LTR has been deleted to make this a self-inactivating (SIN) vector. Upon infection and chromosomal integration into the target cell genome, the SIN deletion is transferred to the 5 LTR, resulting in an integrated expression cassette in which only the CMV promoter is active.
For Research Use Only. Not for use in diagnostic procedures.