RNase Block Ribonuclease Inhibitor is a 50-kDa protein that specifically inhibits common eukaryotic RNases including RNase A, RNase B, and RNase C. It is a recombinant ribonuclease inhibitor derived by a molecular cloning technology that inactivates RNases by binding noncovalently to the RNase molecule.
For Research Use Only. Not for use in diagnostic procedures.
Only 25 to 40 U is required for a standard transcription reaction
Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts with 40 U of RNase Block Ribonuclease Inhibitor for 1 hour at 37°C and by checking for the absence of degradation.
Applications: RNA transcription, In vitro RNA translation, RNA capping, and Any application where eukaryotic RNase contamination is a potential problem
RNase Block does not inhibit RNase H, RNase T1, T7, T3, or SP6 RNA polymerases, Taq DNA polymerase, AMV or MMLV reverse transcriptases
Specifications
Concentration
40 U/µL
Storage Buffer
20 mM HEPES-KOH (pH 7.6), 50 mM KCI, 8 mM DTT and 50 % (v/v) glycerol
Unit Definition
One unit will inhibit 50 % of the activity of 5 ng of RNase A.