BioTek Synergy H1 Multimode Reader
BioTek Synergy H1 Multimode Reader
Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection. Fluorescence bandwidth can be set between 9 and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.
- Multimode Microplate Readers
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Product Details
- Hybrid plate reader provides flexibility and performance with a combination of monochromator and filter optics for any microplate assay and flexible detection capabilities
- Upgradable, modular design allows you to add detection modes, gas control, and dual reagent injectors as your laboratory workflows evolve
- Variable bandwidth settings range between 9 and 50 nm, in 1 nm increments for either increased sensitivity or increased specificity
- Automated Z-focus provides the best performance for all plate types and at low volumes, adjusting reading height to deliver high-performance measurements and high-quality data
- Extended dynamic range enables signal detection across a 7 log measurement range, delivering high sensitivity in low-end measurements and preventing saturated signals in high-end measurements
- Environmental controls and robust shaking profiles enable consistent data in live cell assay workflows, including temperature control to 70 °C and CO2/O2 control
- Dual syringe injectors with specialized tips eliminate the need for regular tubing replacement, and two tip types offer flexible use with straight tips for vigorous mixing and rapid inject/read assays or angled tips that do not disturb cell layers
- Microvolume analysis with Take3 plate measures up to 16 or 48 samples in one run, saving time compared to single-sample devices
Automation Compatibility |
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Environment Control |
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Filter Detection Modes |
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Imager/Reader Options |
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Instrument Control |
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Monochromator Detection Modes |
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Shaking |
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Supported Labware |
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Hybrid plate reader: Flexibility and performance
With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Monochromator: variable bandwidth, absorbance, fluorescence, luminescence Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser.
Learn MoreVariable bandwidth for sensitivity and specificity
With its patented combination of monochromator and filter optics, Synergy H1 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Monochromator: variable bandwidth, absorbance, fluorescence, luminescence. Filters: fluorescence polarization, time-resolved fluorescence.
Learn MoreAbsorbance
Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator.
Learn MoreFluorescence polarization
Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light. Depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not.
Learn MoreFiltered Luminescence
Some luminescence methods call for filtered luminescence. In the Agilent BioTek multimode readers, filters can be used to modulate the luminescence emanating from the sample.
Learn MoreFlash Luminescence Detection
Flash luminescence is the result of a chemical or biochemical reaction, triggered by a rapid injection of a catalyst into the microplate well immediately before detection.
Learn MoreFluorescence intensity
Fluorescence intensity detection has a much broader range of applications than absorbance detection.
Learn More- Key Literature
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- Brochures
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- 23 Nov 2021
- 72.95 KB
- Application Notes
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Optimization of Agilent pH‑Xtra Glycolysis Assay using BioTek Gen5 Software
This guide offers Step-by-step instructions to maximize the functionality of the pH Xtra template (AgilentXpH) for BioTek Gen5, including plate reader setup and signal optimization.
- User Manuals
- English
- 09 Sep 2021
- 1.28 MB
Agilent Synergy H1 Microplate Reader Site Preparation Checklist
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
- Site Preparation Checklists
- English
- 27 Mar 2023
- 200.55 KB
- FAQs
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Agilent Cell Analysis Publication Database
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