BioTek Gen5 Software for Detection
Turn microplate data into meaningful decisions.
- BioTek Gen5 software for BioTek multimode and single mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning and well area scanning. It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications.
- Gen5 microplate reader software is also used to integrate BioTek plate readers to BioTek BioStack and other automated systems.
- <B>All-in-One Reader Software</B>: Controls all Agilent BioTek readers with 150+ quick-start protocols and predefined plate types
- <B>Fast, Flexible Analysis: </B>Provides comprehensive real-time quantitative/qualitative analysis (curve fits, EC/IC50, Z', dose response) with easy Excel export
- <B>Advanced Read Modes:</B> Includes multimode kinetics and area scanning for comprehensive data capture
- <B>Automated Data Optimization:</B> Features like fluorescence spectrum viewing and probe z-height optimization streamline data quality
- <B>21 CFR Part 11 Compliance:</B> Gen5 Secure offers the feature set needed to comply with US FDA 21 CFR Part 11 guidelines
- <B>Automation Compatible: </B>Easy control of instruments like the Agilent BioTek BioStack or use for integration into robotics platforms
Overview
How it works
Integrates with all Agilent BioTek microplate readers
BioTek Gen5 software is an integrated tool for data collection and analysis, compatible with all of the Agilent BioTek multimode and single mode readers. It's easy to get started with more than 150 prepared protocols within Gen5 and online. Gen5 supports endpoint, kinetic, well area scanning, spectral scanning read modes. One-button export sends your data right to Excel, without requiring additional steps.
Powerful data analysis and flexible output options
Gen5 offers extensive data analysis tools for quantitative and qualitative analysis, with multiple curve fits, including 4- and 5-parameter fits, EC/IC50, and Z' calculations. Data transformations, customizable validation and cutoff calculations are all easily defined within the protocol. Gen5 provides quick microvolume results with the Take3 plates, and data from any screen is exportable to Excel at the touch of a single button.
Applications
One software, diverse applications
Gen5 supports applications in diverse labware including assays in microplates, cell culture dishes, and cuvettes. Add the Take3 plate for microvolume nucleic acid and protein quantification. All data acquisition and downstream analysis can be performed in a single software package.
Streamlined reader control and data analysis
Experience seamless control over every Agilent BioTek microplate reader application with Gen5 Software. It supports diverse protocols, including qualitative, quantitative, endpoint, and kinetic assays. Gen5 handles your entire workflow, from capturing data to performing sophisticated analyses like parallel line analysis, EC50 determinations, and spectral scanning, all through its uncomplicated interface.
The GxP solution for 21 CFR Part 11 compliance
BioTek Gen5 Secure provides the essential tools for compliance, including multilevel permissions, electronic signatures, and detailed audit trails. Protect your data and streamline operations with configurable alerts and other key features. We also offer a suite of validation tools and services for both software and hardware to streamline your path to compliance.
- No recurring fees
- Automation ready
- Available validation package
Tools
Customer reviews for BioTek Gen5 Software for imaging and microscopy
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Agilent Cell Analysis webinars
Featured webinars help you stay informed about timely cell analysis research topics.
Training & events
ELISA: Best Practices and Troubleshooting
Enzyme-linked immunosorbent assay (ELISA) is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process.
Frequently asked questions
How do I transfer Gen5 to a new computer?
The easiest way is to use the Gen5 file originally sent with your reader (on CD or USB). If you do not have this file, the latest Gen5 version can be requested from our Customer Care Center or you can review the available versions here . Once you receive the software file, follow the instructions provided. The installation will require that a user with administrator rights be logged in to the PC. Registering the newly installed software will require your Gen5 serial number. This number can be found on the CD or USB case received with your reader. It can also be retrieved from the Gen5 installation on the old computer by navigating to System > About Gen5 from within the software.
If your reader is connected via USB, you will also need to install the referenced USB driver. The driver can be found at this link: https://www.ftdichip.com/Drivers/VCP.htm. The USB cable should be disconnected from the computer before installing the driver.
Can I install Gen5 on a computer not connected to my plate reader?
Yes. The purchase of Gen5 allows installation of the software on multiple computers depending on the version of Gen5. When Gen5 is installed on a computer that is not connected to the plate reader, you should set the System > Reader Configuration as though your exact model BioTek plate reader or imager were attached. You will receive communication error messages because there is no instrument connected. You can cancel out of these messages and proceed to set up new protocols and analyze data collected on another computer. You will not have the ability to validate steps that require a reader (such as minimum interval setting in a kinetic read), but otherwise, the software is fully functional.
What file types can I import into Gen5 software for analysis?
Raw data text files (.txt) can be imported into Gen5 for absorbance, fluorescence, and luminescence.
Grayscale TIFF images can be imported only with Gen5 versions compatible with Cytation and Lionheart. To learn how to import either .txt files or TIFF files, please refer to the Gen5 Help.
For .txt file imports: Use the instructions in “Import Data from an External Source” in the Gen5 Help.
For TIFF file imports: Use the instructions in “Import an Image Set in Manual Mode” in the Gen5 Help.
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