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ZAP-70* (Autostainer Link 48)

Clone 2F3.2

Reacts with ZAP-70 expressed in T cells, natural killer cells, pro/pre B cells but not in normal mature B cells. The antibody is a useful aid for classification of a subset of chronic lymphocytic leukemias (CLL). In CLL, ZAP-70 expression is closely associated with an unmutated configuration of the immunoglobulin heavy-chain variable region (IgV_H) genes (1).

* This product is for in vitro diagnostic use only. The product embodies technology described in US Patent 7,329,502 and pending Canadian Patent Application No. 2,413,475.

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	2F3.2
Code Number	IR653
Immunogen	Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP-70 (7). See package insert for reference(s).
Isotype	IgG2a, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Autostainer Link 48
Species	Mouse Anti-Human
Specificity	In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP-70 (2). In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP-70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples (2). In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein (6). In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed (6). See package insert for reference(s).

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Featured References

Product Description References: 1. Carreras J, Villamor N, Colomo L, Moreno C, Cajal S, Crespo M, et al. Immunohistochemical analysis of ZAP70 expression in B-cell lymphoid neoplasms. J Pathol 2005,205,507-13.

ZAP-70* (Autostainer Link 48)

Product Details

Atlas of Controls

View supported Instructions For Use documents

1. Carreras J, Villamor N, Colomo L, Moreno C, Cajal S, Crespo M, et al. Immunohistochemical analysis of ZAP70 expression in B-cell lymphoid neoplasms. J Pathol 2005,205,507-13.

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