Wilms' Tumor 1 Protein Antibody (Dako Omnis)

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Clone	6F-H2
Immunogen	Truncated human WT1 protein corresponding to N-terminal amino acids 1-181 (1,2).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN₃. Clone: 6F-H2 (1,2). Isotype: IgG1, kappa.
Solutions	Dako Omnis
Species	Mouse Anti-Human
Specificity	Alternate splicing of the WT1 transcript gene results in four distinct mRNA species which are present in different amounts. The predicted molecular weight of the WT1 protein ranges from 46 to 49 kDa depending on the mRNA species however, the WT1 proteins derived from cells migrate at 50 to 55 kDa in SDS-PAGE (4). This antibody recognizes an epitope found in the amino terminal 84 encoded amino acids of WT1. It reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2, frequently found in subsets of sporadic Wilms' tumors. In immunoprecipitation assays, the antibody has been shown to recognize full-length and in vitro translated WT1. It also detects full-length denatured WT1 (55 kDa) in Western blots of WT1 baculovirus-infected cell lysates (1).

Return to top

Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

Support

Instructions for Use: View supported Instructions For Use documents

Promotions

Wilms' Tumor 1 Protein (Dako Omnis)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents