Vimentin is a 57 kDa intermediate filament protein which forms part of the cytoskeleton of vertebrate cells and is characteristically found in cells of mesenchymal origin. The coexpression of intermediate filaments, particularly vimentin and cytokeratin, has been demonstrated in a variety of normal cells/tissues and in neoplastic lesions, necessitating the use of antibodies against other types of intermediate filaments. The antibody is a useful aid for classification of neoplastic tissues of mesenchymal origin.
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Purified vimentin from porcine eye lens (3). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
In Western blotting of purified porcine vimentin, the antibody labels a single band of 57 kDa corresponding to vimentin. When applying whole cell extracts of cell lines expressing vimentin plus glial fibrillary acidic protein (GFAP), and vimentin plus desmin, respectively, the antibody labels specifically the 57 kDa vimentin band. As directly shown by these experiments, the antibody does not react with the two IF proteins most closely related to vimentin, i.e. desmin and GFAP (3). In IHC, the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.