Vimentin is a 57 kDa intermediate filament protein which forms part of the cytoskeleton of vertebrate cells and is characteristically found in cells of mesenchymal origin. The coexpression of intermediate filaments, particularly vimentin and cytokeratin, has been demonstrated in a variety of normal cells/tissues and in neoplastic lesions, necessitating the use of antibodies against other types of intermediate filaments. The antibody is a useful aid for classification of neoplastic tissues of mesenchymal origin.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Frozen
Formalin
HIER
Clone
V9
Code Number
M0725
Immunogen
Purified vimentin from porcine eye lens (3). See package insert for reference(s).
Isotype
IgG1, kappa
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
Solutions
Concentrate
Species
Mouse Anti-
Specificity
In Western blotting of purified porcine vimentin, the antibody labels a single band of 57 kDa corresponding to vimentin. When applying whole cell extracts of cell lines expressing vimentin plus glial fibrillary acidic protein (GFAP), and vimentin plus desmin, respectively, the antibody labels specifically the 57 kDa vimentin band. As directly shown by these experiments, the antibody does not react with the two IF proteins most closely related to vimentin, i.e. desmin and GFAP (3). In IHC, the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.