The very high specificity has been ascertained in immunohistochemistry as well as in indirect ELISA and immunoblotting. Additionally, the specificity has been tested by crossed immunoelectrophoresis using 12.5 µL antibody per square cm gel area against 2 µL human plasma.
This antibody is useful for the identification of plasma cells and related lymphoid cells containing IgM. It is a useful aid for classification of B-cell neoplasia.
Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Specifications
Application
Formalin
HIER
Clone
Polyclonal
Immunogen
IgM isolated from human serum of a patient with Waldenström's macroglobulinaemia.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Solutions
Autostainer Link 48
Species
Rabbit Anti-Human
Specificity
The antibody reacts with the mu-chains of human IgM. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. In crossed immunoelectrophoresis, using 12.5 µL antibody per cm2 gel area against 2 µL human plasma, only one precipitate corresponding to IgM appears. Staining: Coomassie Brilliant Blue.
In indirect ELISA no significant reaction is observed when using human IgG and IgA as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgM.
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