Epstein-Barr Virus, LMP Antibody (Dako Omnis)

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Clone	CS.1-4
Immunogen	Recombinant fusion protein containing sequences of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP (7).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use mixture of four monoclonal mouse antibodies provided in liquid form as tissue culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2 containing 0.015 mol/L NaN₃. Clone: CS.1, CS.2, CS.3 and CS.4. Isotype: IgG1, kappa.
Solutions	Dako Omnis
Species	Mouse Anti-
Specificity	In Western blotting with lysates of EBV-transformed lymphoblastoid cell lines, the antibody labels a major band of 57-66 kDa depending on the virus isolate, sometimes accompanied by a minor band of 50-55 kDa corresponding to full length and truncated form of LMP. The antibody recognizes 20 geographically distinct EBV isolates (7). The antibody cross-reacts with a 43-44 kDa doublet of uncharacterized normal cell proteins in cellular extracts including that from EBV-negative control cell lines (7). The antibody labels a normal EBV-positive lymphoblastoid cell line established by infection of normal resting B-cells with B95.8 virus, whereas the BL2 cell line, established from a rare EBV-negative sporadic BL-patient, as well as other unrelated cell isolates, including tonsillar B and T lymphocytes, fetal liver and thymus, cultured fetal fibroblasts, human leukaemic cell lines HSB2 and K562 and 7 EBV-negative B-cell lines show no labeling, except the cellular doublet proteins (7).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Epstein-Barr Virus, LMP (Dako Omnis)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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