The isolated β-chain of hCG is used for immunization. The antibody cross-reacts with human luteinizing hormone (LH). For immunohistochemical use, the cross-reaction with LH will not cause misinterpretation, and the antibody is well-suited for identification of hCG in trophoblastic elements and is a useful aid for classification of germ cell tumors.
Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Specifications
Application
Formalin
HIER
Clone
Polyclonal
Immunogen
The isolated beta-chain of hCG.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Solutions
Autostainer Link 48
Species
Rabbit Anti-Human
Specificity
The antibody reacts with hCG, and like most other polyclonal antibodies to beta-hCG it also shows a slight cross-reaction (about 4%) with LH (3). Traces of contaminating antibodies to human plasma proteins have been removed by solid-phase absorption.
In crossed immunoelectrophoresis only the hCG precipitation arch appears when using 12.5 µL Dako Code A0231 per cm2 gel area against 10 µL of concentrated urine from pregnant women (15th-16th week of gestation). No precipitation is seen when 12.5 µL concentrated antibody per cm2 gel area is tested against 10 µL of normal human plasma. Staining: Coomassie Brilliant Blue. See package insert for reference(s).
