CD43 is an integral membrane protein typically expressed at high levels on all leucocytes, except most resting B lymphocytes. Antibodies to CD43 may be a useful aid for the classification of low-grade B-cell lymphomas and myeloid disorders.
Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Specifications
Application
Formalin
HIER
Clone
DF-T1
Immunogen
KG1 cells (myeloblastic cell line).
Isotype
IgG1, kappa
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions
Autostainer Link 48
Species
Mouse Anti-Human
Specificity
Western blotting and immunohistochemical reactivity of the antibody is identical with that of CD43-clustered antibodies. Additionally the antibody reacts strongly with COS-7 cells (monkey kidney cell line) transfected with the CD43 gene, while control cells transfected with the vector only are negative (4). The antibody causes rapid and vigorous aggregation among normal leukocytes and among T and myeloid/monocytic cell lines (5). See package insert for reference(s).
