Anaplastic large cell lymphoma (t(2;5) translocation)

CD246, ALK Protein (Autostainer Link 48)

Clone ALK1

Recognizes a formalin-resistant epitope in both the 80 kDa NPM-ALK chimeric and the 200 kDa normal human ALK proteins (1). Normal ALK protein expression is restricted to the central nervous system. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase (2). This antibody is a useful aid for classification of anaplastic large cell lymphoma (ALCL).

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	ALK1
Code Number	IR641
Immunogen	DHFR-ALK recombinant protein comprising mouse dihydrofolate reductase (DHFR) and amino acids 1359-1460 of the full length human ALK protein, corresponding to amino acids 419-520 of the chimeric NPM-ALK protein (1). See package insert for reference(s).
Isotype	IgG3, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Autostainer Link 48
Species	Mouse Anti-Human
Specificity	Anti-Human CD246, clone ALK1, was clustered as anti-CD246 at the 7th International Workshop and Conference on Human Leucocyte Differentiation Antigens (3). In Western blotting of lysate of the t(2;5)+ SU-DHL-1 cell line, the antibody labels a band of 80 kDa corresponding to NPM-ALK protein. The antibody also recognizes a band of 200 kDa corresponding to the ALK protein in the human rhabdomyosarcoma cell line Rh30 that expresses full length ALK protein, and in the 293T cell line transfected with cDNA encoding full length ALK protein. No reactivity was observed when the 293T cell line was transfected with the vector alone (1). See package insert for reference(s).

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Featured References

Product Description References: 1. Pulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 1997,89:1394-404.
2. Morris SW, Kirstein MN, Valentine MB, Dittmer KG, Shapiro DN, Saltman DL, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma (published erratum appears in Science 1995,267:316-7). Science 1994,263:1281-4.

CD246, ALK Protein (Autostainer Link 48)

Product Details

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