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Pepsin

Pepsin is used for the proteolytic digestion of paraffin-embedded, formalin-fixed tissues prior to in situ hybridization procedures, or prior to staining of certain antigens by immunohistochemical methods. The pepsin in each packet is sufficient for preparing 250 mL of pepsin solution for in situ hybridization, or 500 mL of pepsin solution for immunohistochemistry. When used in a staining bath, this volume is sufficient for treating 25-50 slides.

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