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CRISPR Library Cloning Kits

Rapid Generation of Plasmid Libraries from an Amplified Oligo Pool

The Agilent SureVector CRISPR library cloning kit provides reagents for preparation of a lentiviral plasmid library for in vivo expression of guide RNA (gRNA) sequences. Agilent’s SureVector cloning technology uses a unique enzyme blend to enable the assembly of multiple linear fragments of DNA to create a complete plasmid vector in a single-tube reaction. In the SureVector CRISPR Library Cloning Kit, this technology has been adapted to create a method for insertion of a custom CRISPR library into the lentiviral plasmid pSGLenti.

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