xCELLigence RTCA eSight Software
RTCA eSight Software allows users to quickly configure experiments with an intuitive plate map interface. Enter cell type, seeding density, treatment conditions, and concentrations, or apply saved templates for faster setup. Export experiment details in Excel, text, or PDF format with a single click and easily generate videos.
The RTCA eSight Software streamlines simultaneous real-time capture and acquisition of dual impedance and live-cell imaging data, all in the same well. This unique dual-readout approach provides continuous kinetic monitoring without disturbing your cultures, ensuring physiologically relevant results.
Track proliferation curves, cytotoxicity readouts, or migration profiles while experiments are running, and apply saved analysis parameters for consistent data handling across studies.
Choose from a variety of assays—including 2D cell migration and invasion, immunotherapy potency assays, 3D spheroid/organoid analysis, wound healing, dilution cloning, virology applications, drug discovery, and cell-by-cell segmentation—using dedicated software modules that automate calculations and streamline reporting.
Ensure data integrity with built-in 21 CFR Part 11 compliance features, including secure user authentication, full audit trails, electronic signatures, and controlled workflows—supporting both research and GMP environments.
Typical immune cell killing assays are labor-intensive and require users to collect multiple timepoints using a combination of techniques. In contrast, the label-free xCELLigence RTCA instruments provide a complete view of immune cell killing and closely mimics activity in vivo. Obtain highly sensitive, reproducible, and direct measurements of immune cell killing potency in real time. This simple and high throughput workflow also provides greater insight into cellular mechanism of action, serial killing, and exhaustion.
Advance your anti-viral therapy or vaccine development by accurately measuring viral cytopathic effects (CPE) in real time with Agilent xCELLigence RTCA instruments, without the use of labor-intensive plaque assays. Monitor viral CPE automatically, from minutes to days, and greatly reduce workload and manual handling of samples. Measure cell proliferation kinetics, identify the optimal time point for viral infection with different cell seeding densities, and assess virus-mediated cytopathogenicity in one workflow.
Determine cytotoxic effects of drugs or compounds by monitoring cell proliferation, cell size or morphology, and cell-substrate attachment quality in real time. Monitor the kinetics of long-term cellular responses with this simple and high-throughput cytotoxicity assay. Make informed decisions about the timing of cell treatments and easily generate dose-response curves at multiple time points, without increasing the workload.
Learn about apoptosis and proliferation assays that are simple, quantitative, and high throughput to provide multiple perspectives into cell health and viability. Measure real-time cell health directly and capture early and late apoptotic events such as modulation of cell adhesion, cell membrane integrity, and cell detachment. Track proliferation of host cells to detect readout for drug toxicity or presence of a virus. Detect and quantify apoptosis in living cells by label-free approach, or using apoptotic or nuclear labeling reagents. The xCELLigence RTCA eSight provides multiple temporal vantage points (impedance, fluorescence imaging, and brightfield imaging).
The Agilent xCELLigence RTCA eSight Software enables real-time monitoring of 3D cell culture systems, including spheroids and organoids. Researchers can continuously measure and quantify spheroid formation, growth, invasion, and shrinkage in response to treatments. Additional readouts include morphology changes, cell viability, cytotoxicity, immune cell–mediated killing, and migration or invasion metrics. These assays provide multiparametric insights by combining impedance and imaging, allowing both population-level trends and single-cell behaviors to be evaluated over time.
