xCELLigence RTCA eSight Software
RTCA eSight Software
RUO
The Agilent xCELLigence RTCA eSight software drives the Agilent xCELLigence RTCA eSight instrument with flexible assay set up, real-time impedance and imaging acquisition, and integrated analysis tools. The RTCA eSight supports a broad range of labware—including the Agilent E-Plate VIEW 96, standard 96-well plates, and formats from 6- to 384-well plates, dishes, and flasks. It offers both real-time and offline modes for continuous monitoring or post-run analysis. With version 1.4.0, the software introduced support for up to five 96-well regular imaging microplates, 3D organoid workflows, and features to support 21 CFR Part 11 compliance and GMP environments.
The base software provides the core functionality to set up, run, and analyze real-time cell assays. By combining impedance and imaging, it monitors adhesion, proliferation, viability, and cytotoxicity in 2D cultures. Built-in tools such as slope, doubling time, percent of control, and EC50/IC50 calculations ensure reliable, reproducible results.
With version 1.5.0, an optional AI add-on license introduces AI-powered, label-free cell segmentation for automated cell counting, faster analysis, and more reproducible image analysis workflows.
Additional application modules expand these capabilities with immunotherapy assays, 3D spheroid and organoid analysis, single-cell tracking, dilution cloning, and wound healing. This modular design gives researchers the flexibility to study processes ranging from immune cell killing to migration and clonal development.
The base software provides the core functionality to set up, run, and analyze real-time cell assays. By combining impedance and imaging, it monitors adhesion, proliferation, viability, and cytotoxicity in 2D cultures. Built-in tools such as slope, doubling time, percent of control, and EC50/IC50 calculations ensure reliable, reproducible results.
With version 1.5.0, an optional AI add-on license introduces AI-powered, label-free cell segmentation for automated cell counting, faster analysis, and more reproducible image analysis workflows.
Additional application modules expand these capabilities with immunotherapy assays, 3D spheroid and organoid analysis, single-cell tracking, dilution cloning, and wound healing. This modular design gives researchers the flexibility to study processes ranging from immune cell killing to migration and clonal development.
For Research Use Only. Not for use in diagnostic procedures.
Download the product software using the DOWNLOADS button above, or request more information about the RTCA eSight Software
Product Details
Features
- Capture more from a single setup with dual impedance and imaging readouts, multimodal data acquisition, and powerful analysis tools for information-rich results.
- Monitor and quantify cell health, proliferation, cytotoxicity, and apoptosis in real time using simultaneous impedance and imaging.
- Evaluate and compare immunotherapies, CAR-T, NK cells, and antibody treatments with automated immune cell killing and potency assays.
- Expand throughput and accelerate discovery by running up to 5x96 imaging plates simultaneously without hardware changes.
- Model and analyze complex biology with advanced 3D workflows, including spheroid growth, invasion, and patient-derived organoid studies.
- Track and characterize single-cell behavior, clonal populations, and wound healing dynamics through automated cell-by-cell analysis.
- Ensure data integrity and regulatory compliance with built-in 21 CFR Part 11 features like electronic signatures, audit trails, and GMP support
- Reduce image analysis time using AI-driven, label-free cell segmentation for automated cell counting with a one-click workflow
How It Works
Applications
