Automated Imaging Microplate Reader, BioTek Cytation 5

Features

Modular, upgradable imaging modes, plus multimode detection enables a range of cell-based assays and applications beyond a standard microplate reader
Combination of hybrid plate reader and advanced microscopy mode increases productivity for a system that is ready for any assay
Hit-picking capability reduces time and computer memory with no unnecessary data collection or analysis
Fully automated microscopy tasks, from slide scanning to time-lapse live cell assays, and flexible hardware increase productivity and reduce manual labor
Combination of monochromator and filter optics delivers flexibility and performance to microplate assays, and choice of optics allows you to read microplates in a wide range of applications
BioTek Gen5 software enables walk-away automation for your image process and analysis workflows and data analysis tasks
Variable bandwidth quad monochromator optics enhance assay performance including increased sensitivity and lower limits of detection

Specifications


Automation Compatibility	BioSpa BioStack 3rd-party BenchCel
Camera	Sony CMOS, 16-bit color
Environment Control	4-Zone Incubation to 65 °C Condensation Control Peltier Cooling CO2, O2 Control
Filter Detection Modes	BRET Fluorescence Intensity Fluorescence Polarization Luminescence Time Resolved Fluorescence TR-FRET Alpha (Laser)
Imager/Reader Options	Joystick stage control Laser autofocus Reagent injectors
Imaging Methods	Montage Multi-color Single-color Time lapse Z-stacking
Imaging Modes	Widefield fluorescence Brightfield High contrast brightfield Color brightfield Phase contrast
Magnification Range	1.25x to 60x air 4x to 40x phase contrast
Microscope Type	Inverted microscope
Monochromator Detection Modes	UV-Vis Absorbance Fluorescence Intensity (variable bandwidth) Luminescence Time Resolved Fluorescence (secondary)
Shaking	Linear Orbital Double-orbital
Supported Labware	6 to 1536-well plates Slides Cell culture dishes Cell culture flasks (T25) Hemocytometers Chamber slides Take3/Take3 Trio

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How It Works

Applications

Technology

Peltier cooling for environmental stability

The Peltier Cooling Module promotes rapid interior cool down after incubated processes, allowing efficient switching between multiple applications without unwanted temperature changes. For sensitive assays run at room temperature, the Cooling Module maintains environmental stability within Cytation at < 1 °C change.

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Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Monochromator: variable bandwidth, absorbance, fluorescence, luminescence Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser.

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Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

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Proprietary Laser Autofocus

Laser autofocus and image-based (or software-based) autofocus are two methods of autofocus widely used in microscopy.

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Time-resolved fluorescence

Time-resolved fluorescence (TRF) measurement is very similar to fluorescence intensity (FI) measurement. The only difference is the timing of the excitation / measurement process.

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Alpha detection

AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym 'Alpha' stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios.

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Absorbance

Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator.

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Fluorescence polarization

Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light. Depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not.

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TR-FRET

Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore.

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Cell Counting

Accurate cell counting is a critical step across a spectrum of cell biology applications. Direct cell counting uses methods that identify and enumerate individual cells, as opposed to indirect methods that estimate population sizes using biochemical methods.

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Object Tracking

With the incorporation of the object tracking module, available within Agilent BioTek Gen5 software for imaging and microscopy, this can be easily accomplished in a fully automated fashion.

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Automatic Region of Interest Identification

This process enables walk-away slide scanning for four or more slides, if combined with Agilent automation offerings, and can be used with the Agilent BioTek Lionheart automated microscopes and Agilent BioTek Cytation imaging multimode readers.

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Literature

Key Literature

Download Literature

Download a product brochure or data sheet

Brochures
English
23 Nov 2021
72.95 KB
PDF

Quantitative Assessment of Antioxidant Activities in Living Cells Using Cytation 5 Cell Imaging Multimode Reader

In this application note, the Agilent BioTek Cytation 5 cell imaging multimode reader was used for quantitative assessment of antioxidant activities in living cells.

Application Notes
English
12 Apr 2022
1.04 MB
PDF

Expression and Intracellular Translocation of Cancer Biomarkers in Hepatocarcinoma Cells Induced by Changes in Mitochondrial Metabolism

Hepatocarcinoma is one of the most common cancer types in the U.S. with limited treatment options and poor survival rate.

Application Notes
English
08 Nov 2022
1.47 MB
PDF

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