AdvanceBio Gly-X Technology
- N-Glycan Sample Preparation Kits
Product Details
- Four dyes are currently available for the Gly-X platform:
- InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
- 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
- InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.
- APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours.
Videos
- Application Notes
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Comparative Analysis of Agilent AdvanceBio Gly-X N-Glycan Prep with InstantPC Kit
This application note compares the Agilent AdvanceBio Gly-X N-glycan prep with Instant PC kit and Waters GlycoWorks RapiFluor-MS N-glycan kit for N-glycan analysis.
- Application Notes
- English
- 17 May 2023
- 782.74 KB
Agilent Biocolumns - Critical Quality Attributes - Glycan Compendium
Application compendium for glycan analysis.
- Application Notes
- English
- 24 Nov 2021
- 6.73 MB
Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS
Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS
- Application Notes
- English
- 23 Sep 2021
- 1.19 MB
Development of a Rapid 2-AB Sample Preparation Workflow for N-Glycans
The characterization of N-glycans is essential to the development of biotherapeutics.
- Application Notes
- English
- 08 Jan 2021
- 601.12 KB
Preparation of Released N-Glycan Samples from Monoclonal Antibodies Using Agilent AdvanceBio Gly-X 2-AB Express for LC-Fluorescence Analysis
A method using the Agilent AdvanceBio Gly-X 2-AB Express kit and 2-AB labeling for the release of glycans with LC/FLD analysis.
- Application Notes
- English
- 17 Sep 2019
- 880.59 KB
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AdvanceBio Gly-X Technology Frequently Asked Questions
Frequently Asked Questions for Gly-X Technology
- User Manuals
- Publications
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Quick and Efficient Sample Cleanup for Biomolecule Analysis
Learn how to use centrifugation with gel filtration cartridges for sample cleanup. This approach is a rapid, convenient, straight-forward technique that can be used to easily avoid the problems associated with analysis of formulated monoclonal antibodies.
- 01 Apr 2024
The Importance of Glycan Analysis and Characterization for Biopharma
Professor Michael Butler, head of the National Institute for Bioprocessing Research and Training (NIBRT) Cell Technology Group (CTG), explains that the characterisation and analysis of glycans is important for the biopharma sector as it can help in the development of therapeutic agents, such as monoclonal antibodies and other recombinant proteins. These are used in therapies in a range of serious conditions including cancer, inflammatory diseases, and cardiovascular diseases. The mechanism of action depends upon their strong affinity to molecular targets associated with disease.
- Drug Discovery World (DDW)
- 10 Oct 2022