Be Agilent Sure of Your Biologic's Critical Quality Attributes

Amino Acid & Cell Culture Analysis

Composition of cell culture media is critical to the quantity and quality of the biotherapeutic produced.

LC/UV with sample derivatization is a simple and robust approach to amino acid analysis.

An LC/MS-based workflow allows you to bypass sample derivatization and simultaneously detect other cell culture metabolites.

Intact & Subunit Analysis Using Reversed Phase

Biotherapeutic proteins are often heterogeneous, containing a mixture of fragments and other product-related impurities.

AdvanceBio RP mAb was specifically designed with the optimum pore size for monoclonal antibodies.

The polymer-based chemistry of PLRP-S gives excellent peak shape under formic acid conditions for MS detection.

Intact Analysis Using Hydrophobic Interaction

AdvanceBio HIC columns are designed to quickly determine post translational modifications without compromising performance.

A single chemistry is suitable for multiple applications. The chemistry is optimized to provide increased resolution and peak shape with enhanced selectivity to measure oxidation and drug to antibody ratios.

Aggregate & Fragment Analysis

Aggregation may trigger undesirable immunogenic response, making it imperative to accurately measure aggregation levels.

The inert chemistry of AdvanceBio SEC column gives excellent peak shape without mobile phase additives, ensuring the integrity of your measurement.

Charge Variant Analysis

Charge variants, especially in the complementarity determining region of mAbs, can significantly impact efficacy.

Agilent Bio mAb columns are specially designed for separation of monoclonal antibody charge variants.

Agilent Bio IEX is available in four chemistries to meet any ion exchange need.

Peptide Mapping

Peptide mapping is used to confirm primary sequence, as well as identify and quantify critical PTMs.

The AdvanceBio Peptide Mapping column offers robust performance for a wide range of proteins.

AdvanceBio Peptide Plus offers ideal peak shape under formic acid conditions for MS detection.

Glycan Analysis

As glycans are instrumental in cellular signaling, a protein’s glycosylation can influence the effectiveness of the drug product.

Agilent AdvanceBio Glycan Mapping provides robust HILIC separations for both UPLC and HPLC instrument platforms.

Titer Determination (Affinity)

Quantitation of a monoclonal antibody via affinity chromatography is used to determine when the protein is ready to harvest, or during development of optimal cell culture conditions.

Various antibodies have different affinity for either Protein A or
Protein G.

New Application Compendium