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DNF-930-K0500
Fragment Analyzer System CRISPR Kits
The CRISPR Discovery Gel kit for the Agilent Fragment Analyzer systems automates the screening of heteroduplex assays and provides fragment sizing and relative concentration. These important pieces of information are automatically processed by ProSize data analysis software to indicate successful editing events and other crucial information like percent mutated.
- Fragment Analyzer Systems DNA Analysis Kits
Product Details
- Separate Heteroduplex Cleavage Fragments - Clear sizing and relative quantification data help you determine CRISPR gene editing success
- Powerful Data Analysis - Plugins for ProSize data analysis software automatically identify samples with CRISPR mutation events and provide important information such as percent mutated
- ssDNA Assessment - Quality analysis of ssDNA and detection of incomplete dsDNA digestion aids in determining CRISPR/Cas homology directed repair (HDR) success
- Low sample volume - Only 2 µL of sample required minimizing amount of digestion reaction volume required
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- Key Literature
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Build Consistency into Your Lab’s Workflow with the Agilent Automated Electrophoresis portfolio.
The automated electrophoresis instrument portfolio includes systems for objective assessment of sample integrity, concentration, and fragment size. Download this eBrochure for an interactive experience.
- Brochures
- English
- 03 Jan 2023
- 10.96 MB
Nucleic Acid Analysis for Sample Quality Assessment Using the Agilent Fragment Analyzer Systems
The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable quality control (QC) of nucleic acids for a broad variety of applications, including next-generation sequencing (NGS) libraries, RNA samples, PCR amplicons, and CRISPR workflows.
- Application Notes
- English
- 03 Feb 2022
- 3.76 MB
Analysis of Long ssDNA for Use in CRISPR/Cas HDR with the Agilent 5200 Fragment Analyzer System
The high sensitivity and resolution of the 5200 Fragment Analyzer system allows for detection of small amounts of undigested dsDNA.
- Application Notes
- English
- 18 Feb 2019
- 1.85 MB
- Application Notes
- Brochures
- Data Sheets
- User Manuals
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Agilent CRISPR Discovery Gel Kit Quick Guide for the Fragment Analyzer Systems 96-Capillary Array
This Quick Guide lists the necessary steps to analyze DNA samples with the Agilent CRISPR Discovery Gel kit and the 5300 and 5400 Fragment Analyzer systems.
- User Manuals
- English
- 17 May 2019
- 345.36 KB
Agilent CRISPR Discovery Gel Kit Quick Guide for the 5300 Fragment Analyzer System 48-Capillary Array
This Quick Guide lists the necessary steps to analyze DNA samples with the Agilent CRISPR Discovery Gel kit and the 5300 Fragment Analyzer system.
- User Manuals
- English
- 17 May 2019
- 339.22 KB
Agilent CRISPR Discovery Gel Kit Quick Guide for the 5200 Fragment Analyzer System
This Quick Guide lists the necessary steps to analyze DNA samples with the Agilent CRISPR Discovery Gel kit and the 5200 Fragment Analyzer system.
- User Manuals
- English
- 17 May 2019
- 337.75 KB