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Automated Electrophoresis Software | Agilent


Keep your instrument in peak performance and ensure you have the most up-to-date analysis tools with easy and fast access to the latest updates and versions of operational software.

ProSize data analysis software

This latest version provides users with over a dozen new and improved features, such as:

  • Select the concentration/molarity units that fit your research best: ng/μL and nmole/L, or pg/μL and pmole/L
  • Determine sample molarity based on Peak Maxima or Average Size of a smear
  • Determine % concentration based on molarity or concentration
  • Set the Y-axis units to molarity with a simple click
  • Specify the start time for data to display on the X-axis
  • Easily load your most recent runs
  • Use the DNA Quality Number (DQN) in DNA and NGS analysis modes for fast QC analysis of samples
  • Expand electropherogram image
  • Adjust gel/electropherogram sizes during analysis
  • Analyze a specific portion of an electropherogram using Inclusion Regions
  • Add annotations to the digital gel image and/or the electropherogram
  • Add your company logo to your reports
  • Export images that are easily adjustable to high resolution for publication work
ProSize data analysis software

The ProSize data analysis software package is now available for download.

View ProSize Presentation

Operational software

Download the latest operation software updates and method files for your instrument.

5200/5300 Fragment Analyzer systems
Download Updater Download Method files
5400 Fragment Analyzer system
Download Updater Download Method files

Custom methods and configuration files

FFPE DV200 calculation method/configuration file

A quality metric developed by Illumina for ProSize. Provides unmatched assessment of formalin-fixed, paraffin-embedded (FFPE) RNA quality prior to sequencing through analysis of FFPE RNA tissue before running in TrueSeq.

Standard Sensitivity RNA kit

For use with p/n DNF-471

High Sensitivity RNA kit

For use with p/n DNF-472

CRISPR plug-in configuration file

The CRISPR plugin can be used for calculating percent cleavages from endonuclease assays. Two different modes allow for the translation of percent cleaved to percent mutated and predicted diploid zygosity when used with the CRISPR Discovery Gel kit.


CRISPR plug-in


Custom methods and configuration files

Updates to the Genomic DNA 165 kb kit (FP-1002)

Two updated method files are available. The Method D Flush restores the shape and signal of the 165 kb peak if it changes after repeated runs. The 20 minute Conditioning method is recommended as a daily conditioning step to improve separations of High Molecular Weight gDNA samples.

Method D Flush 0.5 N NaOH

Restore the shape and signal of the 165 kb peak

20 Minute Conditioning Method

Improve separations of High Molecular Weight gDNA samples