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SureMASTR Technology

The ready-to-use SureMASTR assays are amplicon-based catalog panels aimed at analyzing the genes that matter. The simple workflow reduces processing time and effort, allowing your lab to achieve a smooth daily routine and maximize cost-effectiveness.

Agilent’s SureMASTR (Multiplex Amplification of Specific Targets for Resequencing) catalogue assays offer multiplexed PCR primer mixes with a unique primer design algorithm, enabling enhanced target amplification of DNA. The combination of the assay’s targeted gene selection and the unique primer design algorithm guarantee simultaneous PCR amplification of several 100 amplicons within a single PCR reaction, making your workflow more efficient. By using an amplicon amplification, test results are more accurate, increasing the reliability of your lab testing.

Multiplex PCR

Multiplex PCR is a molecular biology technique for the amplification of multiple genomic targets in a single PCR experiment.

Agilent applies highly multiplexed PCR to develop SureMASTR assays that greatly enhance the overall efficiency and application of genetic testing that can be operated on standard instrumentation.

The Multiplex PCR technology results in highly efficient, low-cost assays enabling a wide range of clinical and diagnostic applications.

Every assay benefits fully from the main advantages of multiplex-based PCR, such as reduction in turn-around time and cost efficiency.


SureMASTR assays (Multiplex Amplification of Specific Targets for Resequencing) offer an innovative combination of premixed PCR primers in a ready-to-use format, enabling enhanced target amplification for DNA-based diagnostics. The technology serves as front-end amplification for sequence analysis for all Next-Generation Sequencing (NGS) systems. It is based on “target amplification” rather than classic target enrichment.

SureMASTR consists of a simple 2-step PCR protocol:

Step 1: Multiplex PCR – enabling specific amplification of the regions of interest

Step 2: Universal PCR – incorporation of molecular barcodes (multiplex identifiers or MIDs) This second PCR step links each read to the sample it originated from.