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Multiplex Amplification of Specific Targets for Resequencing (MASTR)

  • MASTR assays offer an innovative combination of premixed PCR primers in a ready-to-use format, enabling enhanced target amplification for DNA-based diagnostics.
  • MASTR assays serve as front-end amplification for sequence analysis for all Next-Generation Sequencing (NGS) systems. The technology is based on “target amplification” rather than classic target enrichment.

The MASTR assays consists of a simple 2-step PCR protocol:

Step 1: Multiplex PCR – enabling specific amplification of the regions of interest

Step 2: Universal PCR – incorporation of molecular barcodes (multiplex identifiers [MIDs]) This second PCR step links each read to the sample it originated from.