2D-LC Software
- As a part of Agilent InfinityLab two-dimensional liquid chromatography solutions, 2D-LC software allows you to run heart-cutting 2D-LC analysis, including high-resolution sampling and comprehensive measurements. It helps you to increase resolution for co-eluting compounds, analyzing complex samples, or sample matrices.
- This 2D-LC analysis software is easy to use. It simplifies the entire process and helps you smoothly go through the different steps of heart-cutting and comprehensive 2D LC measurements: start with your 1D separation and increase resolution by choosing spots of interest for increased resolution or by re-analyzing your complete sample with different separation conditions in a single run.
- The 2D-LC software also provides comprehensive data analysis and reporting capabilities, and can analyze multidimensional data qualitatively or quantitatively - easily yet powerfully. Analyze and use 2D-LC/MS data for the most extensive information about your samples.
- Set up 2D-LC methods with a few mouse clicks using a graphical 2D-LC preview. Choose between heart-cutting or comprehensive 2D-LC modes. Optionally, start with a previously acquired 1D chromatogram, select spots or ranges of interest, and draw a 2D gradient.
- Run multiple heart-cutting, high-resolution sampling, and comprehensive 2D-LC measurements.
- Display cut chromatograms and contour plots for comprehensive 2D data.
- Analyze 2D-LC data by simple navigation through first- and linked second-dimension chromatograms. Get all the information you need from your sample as qualitative results, including spectral data or reproducible quantitative results.
- Automate valve programming with Agilent 2D-LC instrument control, which eliminates the need for manual interaction. Separation in the first and second dimension are completely independent when using Agilent multiple heart-cutting valves, for the highest storage capacity and fastest parallel analysis.
- Maximize the available 2D separation space for highest peak capacity and fastest analysis with shifted gradients, which can be edited graphically or numerically.
- Use time-based peak parking for known samples, and peak-based or dynamic peak parking for unknown samples (or when handling variable first-dimension retention times).
- Select dedicated flush gradients for fast analysis and minimum carry-over.
- Take advantage of Agilent active solvent modulation in your methods to improve second-dimension resolution and sensitivity by diluting strong first-dimension solvents.
- Report your two-dimensional data and results.
- Apply custom calculations for advanced third-level data analysis.
- Get GC Image LC x LC Edition Software for UV and single quadrupole or Q-TOF/QQQ detection from Agilent. Visualize your 2D data and use sophisticated data analysis for comprehensive 2D-LC data, qualitative and quantitative results, and statistical analysis.
- Get dedicated 2D-LC software training and services for the highest productivity in your lab.
Overview
How it works
Method setup in 2D-LC Software for OpenLab CDS and MassHunter
With Agilent 2D-LC software, you can set up methods with a few mouse clicks:
- Choose between heart-cutting or comprehensive 2D-LC modes.
- Optionally start with a previously acquired 1D chromatogram, and select spots or ranges of interest for improving resolution.
- Interactively draw a second-dimension gradient or edit it numerically. Maximize the available 2D separation space with shifted gradients for the highest peak capacity and fastest analysis.
- Refine method parameters for precisely cutting peaks or scheduling them for analysis.
Data analysis and reporting for heart-cutting 2D-LC
Analyze even your most complex samples with powerful data analysis in Agilent 2D-LC software for OpenLab CDS. Simply navigate between first-dimension chromatograms and related second-dimension chromatograms and numeric results. Visualize two-dimensional results in a contour plot, which is linked to chromatograms and tables. Using 2D data analysis methods, automatically identify and label compounds in 2D retention time space. Use MS spectra for qualitative measurement results. Use 1D and 2D results for quantitative data analysis.
Reporting 2D-LC results with 2D-LC Software for OpenLab CDS
2D-LC software for OpenLab CDS is targeted to 2D-LC measurements with UV or single quadrupole detection. It offers easy method setup, 2D-LC instrument control, sophisticated 2D data visualization, analysis, and advanced reporting. Compliance features of OpenLab CDS like 2D system suitability tests, user privileges, access control, and audit trails enable the use of 2D-LC in quality control labs. Import 2D methods and data from 2D-LC software for ChemStation.
Applications
2D-LC Software for OpenLab CDS
2D-LC software for OpenLab CDS is targeted to 2D-LC measurements with UV or single quadrupole detection. It offers easy method setup, 2D-LC instrument control, sophisticated 2D data visualization, analysis, and advanced reporting. Compliance features of OpenLab CDS like 2D system suitability tests, user privileges, access control, and audit trails enable the use of 2D-LC in quality control labs. Import 2D methods and data from 2D-LC software for ChemStation.
2D-LC Software for MassHunter
This integrated software for Q-TOF and triple quadrupole detection in MassHunter includes the full suite of 2D-LC method setup and development capabilities for heart-cutting and comprehensive 2D-LC, plus instrument control for the 2D-HPLC system. Cut markers indicate the relation of first-dimension chromatograms to second-dimension chromatograms and data. Second dimension chromatograms with spectral MS and UV data can be displayed and analyzed in MassHunter Qualitative Analysis, Quantitative Analysis, and BioConfirm software.
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News
Agilent introduces the Infinity III LC Series, transforming HPLC technology
Agilent Technologies launched the Infinity III LC Series, including the 1290 Infinity III LC, 1260 Infinity III Prime LC, and 1260 Infinity III LC systems. The systems feature InfinityLab Assist Technology for automation of daily HPLC tasks, system troubleshooting, and diagnostics. The new systems are designed to improve lab efficiency and reduce errors, promoting sustainability with My Green Lab’s ACT Ecolabel 2.0.
21 Oct 2024
Agilent Wins Two Scientists’ Choice Awards for Best New Analytical Product of 2024 and Excellence in Analytical Science Communication
Agilent Technologies Inc. received two prestigious Scientists’ Choice Awards: 2024 Webinar of the Year for “HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series” and Best New Analytical Product of 2024 for the Agilent 7010D Triple Quadrupole GC/MS System. These awards, voted on by the global scientific community, recognize Agilent's leadership in innovative technologies and impactful scientific education.
03 Jun 2025
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